This study present a new record of bat distribution in Guangdong province, China. 4 small female bats were collected by harp traps in evergreen forest mixed with sparse bamboo in Nature Reserve of Guangdong Fengkai Heishiding in April, 2014. After checked the bats’ characteristics of pelage colour, external morphology, skull and dental, they were identified as Titania’s woolly bat (Kerivoula titania). The dorsal surface hairs are greyish, with black in basal, light grey in mid-parts and dark grey on tips. The ventral surface hairs are black-brown, with black in roots and grey-brown on tips. External morphology with funnel-shaped ears and long-lanceolated tragus, no extra noseleaf structure. Small body with 32.30 ~ 34.11 mm of forearm length and 16.47 ~ 17.72 mm of tibia length, flattened skull with 13.99 ~ 14.59 mm of greatest length and 4.66 ~ 5.17 mm of height. The dental formula of these specimens is 2.1.3.3/3.1.3.3 = 38 (Fig. 1, Fig. 2, Table 1). Use Petterson D500x (Sweden) to recording the bats echolocation calls in free flying status indoor and analysed on BatSound v.4.1.4. Use SPSS v.19 to count the analysis results. Their echolocation calls were frequency modulated (FM) with high main frequency 114.3 ± 3.9 kHz, long frequency bandwidth 117.8 ± 12.3 kHz, duration time 1.7 ± 0.3 ms and interval time 12.9 ± 1.3 ms (Fig. 3, Table 2). It is the first time to describe the echolocation calls of this species. The discovery of K. titania in Nature Reserve of Guangdong Fengkai Heishiding represent a new record in Guangdong province, China. These specimens were preserved in School of Life Sciences, Guangzhou University.