The activity pattern of wild animals are influenced by both the external environment and the internal physiological mechanism. The research on the activity pattern of wild animals is one of the bases of scientific conservation. Daily activity rhythm and activity intensity of six reintroduced Chinese water deer (Hydropotes inermis) have been studied by radio-telemetry in Songjiang Punan Woodland and Shanghai Binjiang Forest Park. Through radio tracking, individuals were located by the walk-in technique. All movement fixes were processed by Locate III and ArcGIS Map 9.2. We analyzed the impact of human activities on the activity pattern of the deer. Kolmogorov-Smirnov test was used to test the normality of data and Mann-Whitney U test was used to compare the mean activity intensity of different individuals, day and night, and different seasons. The results showed that in Songjiang Punan Woodland, the average activity intensity of the three deer was 144.88 m/h. The activity intensity in the daytime was slightly higher than that at night, and decreased from summer to the next spring (Table.1).,The activity intensity of spring was significantly lower than other seasons by Mann-Whitney U test. The activity intensity of June was the highest and March was the lowest (Fig.1). The individual mean activity intensity of the deer also decreased from June to March (Fig.2). Two obvious activity peaks were found in the morning (5:00 ~ 8:00) and the evening (18:00 ~ 21:00). Another small peak was found after noon (13:00 ~ 15:00) (Fig.3 and Fig.4). The small active peak before dawn could only be found in autumn. The deer showed a clearer crepuscular behaviour in summer and autumn (Fig.5). In Shanghai Binjiang Forest Park, the average activity intensity of the three deer was 69.90 m/h. The activity intensity in the daytime was slightly lower than that at night (Table.2). It was significantly higher in winter than in spring, and decreased from December to May (Fig.6 and Fig.7). The deer didn’t show clear crepuscular behaviour (Fig.8). The activity peak was only found near midday (9:00 ~ 11:00), especially for the male in winter (Fig.9 and Fig.10). It was assumed that artificial feeding, tourists and other human activities had influenced the activity pattern of the released deer.