In order to investigate the blood gas, acid-base balance and electrolyte characteristics of blood in subterranean rodents under normal oxygen condition, these parameters were messured by the i-STAT blood gas analyzer in Gansu zokor (Myospalax cansus), Plateau zokor (Myospalax baileyi) and SD Rat (Rattus norvegicus) which had adapted at normal oxygen condition for 4 weeks. The comparison of difference between groups were analyzed with one - way ANVOA method using SPSS 17.0. The research showed that there were no significant difference of PaO2, PvO2, PaO2-PvO2, SaO2, SvO2 and oxygen utilization rate between Gansu zokor and Plateau zokor. Their PvO2 and SvO2 were significantly lower than that in SD Rat, but oxygen utilization was significantly higher than that in SD Rat. There were no significant difference of Hba, Hbv, HCTa and HCTv between Gansu zokor and Plateau zokor. But their HCTa and HCTv were significantly lower than that in SD Rat (Table 1). Blood acid-base characteristics in Gansu zokor and Plateau zokor were different from SD Rat, but the characteristics in Gansu zokor and Plateau zokor were similar. pHa-pHv, TvCO2-TaCO2 in Gansu zokor and Plateau zokor were higher than that in SD Rat. PaCO2 in Gansu zokor and Plateau zokor was significantly lower than that in SD Rat. PvCO2-PaCO2 in Gansu zokor and Plateau zokor was significantly higher than that in SD Rat (Table 2). There were no significant difference of Na+, K+, iCa2+, Na+v-Na+a, K+v-K+a and iCa2+v-iCa2+a in Gansu zokor and Plateau zokor. K+a in Plateau zokor was lower than that in SD Rat, but K+v-K+a in Plateau zokor was higher than that in SD Rat. K+v-K+a, iCa2+a and iCa2+v in Gansu zokor were higher than that in SD Rat (Table 3). The result prompted that the blood gas characteristics, acid-base balance and electrolyte characteristics in Gansu zokor and Plateau zokor were similar, but they are different from SD Rat. The oxygen utilization rate in Gansu zokor and Plateau zokor were higher than that in SD Rat. In order to guarantee internal environment and homeostasis, Gansu zokor and Plateau zokor had strong tolerance to acid-base variation and maintenance to electrolyte stability.