Five specimens of odour frogs, collected from Sanquan town of Nanchuan District in Chongqing from July to August 2014, were identified as Yizhang odorous frog (Odorrana yizhangensis) based on morphological, DNA sequence, and genetic distance analysis, which is new to Chongqing. Morphological comparisons between the specimens from Nanchuan district in Chongqing and that from type locality of O. yizhangensis are as follows: female frogs have more obvious digital subarticular tubercles than males; there are outside metacarpal tubercle, entire web of toes and clear digital subarticular tubercles; the joint tibio-tarsal is over the snout ends when the legs are stretched against the edge of the body. The Nanchuan district of Chongqing is about 5 longitudes and 5 latitudes apart from Yizhang, Hunan, which is the westernmost point of the distribution of O. yizhangensis. Future studies should be focused on whether the morphological, genetic differentiation and the pattern heterogeneity of O. yizhangensis are affected by the special geographic effect of alpine valley of western Yunnan, mountain areas of Nanling in Guangdong, Guangxi, Hunan and Jiangxi, border region of Chongqing, Hubei, Hunan or the climate fluctuations during the quaternary glacial period.