We carried out a survey to understand avian influenza virus infection (AIV) status in the captive wild birds farms at Eastern Dongting Lake, China. We collected 1 997 environmental samples, including fresh fecal and water samples, from 4 captive wild birds farms in the Eastern Dongting Lake area from November 2011 to October 2013. Environmental samples were tested for the presence of avian influenza virus using quantitative PCR assays and virus isolation techniques. The results were analyzed using SPSS and excel software. AIV prevalence of partly samples was 36.62%, and it was the highest (47.83%) in March 2012 (figure 2). The positive rate of AIV from fecal samples and water samples was 40.45%, 30.19% (table 1), respectively, and there is no statistical difference between the both by χ2 test. We isolated 26 and 2 AIVs from the fecal samples and drinking water, respectively, including H10, H4, H5, and N7, N8. Most AIVs were in November and December (figure 1), from fecal samples of wild ducks in Junshan district primarily (table 2). The AIVs were found from the environmental samples in the captive wild birds farms, and the measure that monitoring AIV in Dongting lake throughout the year should be implemented in.