To study the response properties of constant frequency-constant frequency (CF-CF) combination sensitive neurons in inferior colliculus (IC) of pratt’s roundleaf bat (Hipposideros pratti), and the proportions of facilitatory and inhibitory CF-CF combination sensitive neurons in high frequency representation region of IC, single CF tones with different frequencies and intensities and double CF tones with different interpulse intervals were used to study the responses of combination sensitive neurons in IC. The responses of 77 IC neurons of 7 bats with normal hearing were recorded by intracellular recording method. All data obtained under different stimulation conditions were processed and plotted using Sigmaplot 10.0. Among 77 recorded neurons，37 (48.1%) of them were the CF-CF combination sensitive neurons. Most neurons (24/37) were the inhibitory CF-CF combination sensitive neurons (Fig. 2B) and 13 were the facilitatory (Fig. 2A). These results indicated that there were both facilitatory and inhibitory CF-CF combination sensitive neurons in IC of pratt’s roundleaf bat. And the proportion of inhibitory CF-CF combination sensitive neurons was larger than facilitatory. The CF-CF combination sensitive neurons may play an important role in temporal and spectral integration of information in the echoes from the targets during the navigation of bats.