Cold stress for the study of different temperature on the Altay Sheep (Ovis aries) cell-mediated immunity and heat shock protein 70( hsp70). Collected before and after the test cold stress Altay Sheep liver, lung, spleen, lymph node tissue and serum, using real-time quantitative PCR maximum second derivative method (2-ΔΔCt) for each tissue expression of hsp70 were statistically analyzed, while using Enzyme linked immunosorbent assay (ELISA) method for determining cold stress before and after the change of interleukin -4 (IL-4) and interleukin -2 (IL-2) concentration in the serum. The results showed that the expression of various tissues Altay Sheep heat shock protein hsp70 have increased in cold conditions, especially large increase in the expression of the magnitude of spleen tissue. ELISA method for determining the concentration of IL-4 Altay Sheep occurs after cold stimulation decreased significantly (P = 0.016), and IL-2 after cold stress did not change significantly (P = 0.502), a slight reduction. Studies have shown that, under conditions of cold stress, high levels of hsp70 stress to protect the body from injury. And in the process of cold stress, the body's immune system is suppressed.