From April to August in 2010,we studied the reproductive ecology of Lanius isabellinus isabellinus in An’xin Extra-arid Desert National Nature Reserve. Breeding season lasts from the end of April to Early August. There were 3-6 pieces of eggs per nest, the average clutch size of L. isabellinus was 4.67±0.57(n=21)eggs and the egg volume was 3.14±0.32 cm3 (n=95), average fresh eggs mass was 3.48±0.20 g (n=20). After laid the first egg, the female birds began incubation, and males were responsible for courtship feeding and guarding. Measured by automatic temperature recorder, the average incubation temperature was 38.19±0.77℃, the duration of incubation was 15-16 days. Nestling period lasted 12-15 days, after nestlings left, the parents birds continued to feed the baby birds, the whole breeding period was up to 31 days at most. Hatching success was 82.50%(n=80), nesting success was 46.94%, fledging success was 62.96%(n=81), nest success rate was 58.62%. During the entire breeding season, three nests of L. isabellinus were found to be parasitized by Cuculus canorus, which accounting for 10.34% of the total nests.