One female specimen of Brachytarsophrys chuannanensis was collected when conducting resources investigation of amphibious and reptiles in Longquan temple, Heiwan Riverin, Jiangkou county (27°51′06″N, 108°41′06″E, elevation 559 m) of Fanjingshan National Nature Reserve from 24-31 July 2013. The species is a new record of amphibians in Guizhou Province, China. It was preserved in Guizhou provincial key and special laboratory for biodiversity and ecology appliance of Guiyang University.