Neuropeptides are small protein-like molecules excreted by neuroendocrine tissues.Recently, the economic value of cephalopods is highly awared by the public, and the research on cephalopods neuropeptides is widely conducted.Using techniques of high performance liquid chromatography (HPLC) to isolate and purify, mass spectra (MS) to identify, and immunocytochemistry to target neuropeptides, they are found to play an important role in reproductive regulation.These neuropeptide studies will provide important data for supporting the cephalopods artificial breeding.This review updates the research findings about seven groups of cephalopods neuropeptides including their classifications, locations, structures, and functions, as well as their development application prospectives.