[Objectives] Migration stopover is an important process in the life history of waterbirds. Changes in hydrology and anthropogenic factors directly affect the sustainable use of migration stopovers by waterbirds. [Methods] We counted the stopover number and duration during migration season of Oriental Stork (Ciconia boyciana) in Longfeng Lake Wetland Provincial Nature Reserve, Changling County, Jilin Province from 2015 to 2020, and recorded water level and human disturbance types, then analyzed the impacts of habitat factors on the migration dynamics of Oriental Stork by means of correlation analysis, differential analysis and general linear model. [Results] The results show that Longfeng Lake Wetland Provincial Nature Reserve is an important stopover site for Oriental Stork, the stopover duration (Z = 17.500, P = 0.937) and number (Z = 0.890, P = 0.580) in spring and autumn are basically same (Table 1); the increased water area resulted in the decrease of stopover duration of Oriental Stork (F = 5.119, P = 0.038); the effect of tourism activities on Oriental Stork’s stopover number was significantly greater than vehicle (Z =﹣2.783, P = 0.005) and photography (Z = 1.679, P = 0.007) disturbance, which indicated the former have more disturbance more than the latters for waterbirds. [Conclusion] This study suggest that the managers of waterbird stopovers adjust the hydrologic changes reasonably, reduce the interference of human activities, which is significant to strengthen the management and protection of endangered waterbirds such as Oriental Storks.