Abstract:Four species of hypotrichous ciliates isolated from farmland soils in Gansu, Cyrtohymena citrina (Fig. 1 and 2), Gastrostylides dorsicirratus (Fig. 3 and 4), Vermioxytricha arenicola (Fig. 5﹣7) and Hemisincirra sp. (Fig. 8 and 9), were studied morphologically using non-flooded Petri dish method, live observation and protargol staining methods. Among the species, C. citrine, G. dorsicirratus and V. arenicola are new record species of ciliates in China and H. sp. is kept as an unnamed form owing to the lack of some key taxonomic data and morphogenetic as well molecular information, which are required for accurate identification. Their morphological character were described in details and discussed.