重庆发现水城角蟾及其系统发育分析
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1.吉首大学生命科学学院 吉首 416000;2.吉首大学杜仲综合利用技术国家地方联合工程实验室 张家界 427000;3.两栖爬行动物爱好者

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黄杰,男,硕士研究生;研究方向:两栖爬行动物分类与保护;E-mail:2993171853@qq.com; 肖扬,男,硕士研究生;研究方向:两栖动物保护;E-mail:1536413151@163.com。

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基金项目:

国家自然科学基金项目(No. 32060128),吉首大学科研项目(No. Jdy25029);


Boulenophrys shuichengensis found in Chongqing and its phylogenetic analysis
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1.College of Life Sciences, Jishou University, Jishou 416000; 2.National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides, Jishou University, Zhangjiajie 427000, China; 3.Enthusiast of Amphibians and Reptiles

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    摘要:

    2025年6月16日于重庆市南川区三泉镇采集1尾布角蟾属(Boulenophrys)标本(标本号:JSU HJ2025005),经形态比较与分子系统发育分析,确定该标本为水城角蟾(B. shuichengensis),系重庆市两栖类分布新记录种。基于线粒体16S rRNA和COI基因序列该标本与来自贵州省大方县的水城角蟾的遗传距离分别为0.00%和0.98%。基于线粒体16S rRNA、12S rRNA和COI基因联合构建系统发育树,揭示水城角蟾与尾突角蟾(B. caudoprocta)互为姐妹种关系,并与荔波角蟾(B. liboensis)、淡肩角蟾(B. boettgeri)和慕阜山角蟾(B. mufumontana)具有较近缘的系统发育关系而聚为一支。水城角蟾新分布点的发现,提升了对重庆市两栖动物物种多样性的认识,同时可为该物种分类、生物地理演化研究以及保护提供新的参考资料。

    Abstract:

    On June 16, 2025, a specimen of the genus Boulenophrys (voucher number:JSU HJ2025005) was collected in Sanquan Town, Nanchuan District, Chongqing. Through morphological comparison and molecular phylogenetic analysis (Table 1, Figs. 1 ~ 3), the specimen was identified as Boulenophrys shuichengensis, representing a new amphibian species record for Chongqing. The calculated uncorrected p-distances between this specimen and B. shuichengensis from Dafang County, Guizhou Province were 0.00% and 0.98% based on the mitochondrial 16S rRNA and COI gene sequences, respectively (Tables 2 and 4). A phylogenetic tree reconstructed from the combined mitochondrial dataset (16S rRNA, 12S rRNA, and COI genes) revealed that B. shuichengensis formed a sister relationship with B. caudoprocta,and then the two species clustered into a clade with B. liboensis, B. boettgeri, and B. mufumontana, indicating their close phylogenetic relationships (Fig. 3). The discovery of this new distribution record of B. shuichengensis enhances our understanding of the amphibian diversity in Chongqing and provides valuable new data for future research on the taxonomy, biogeography, and conservation of this species.

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黄杰,肖扬,吴佩琪,李盼,董佳鑫,张方鹏,周贵评,黄兴龙,张佑祥,吴涛,蒋万胜. 2026.重庆发现水城角蟾及其系统发育分析. 动物学杂志, 61(5): 731-741.

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  • 收稿日期:2025-08-15
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  • 在线发布日期: 2026-10-29
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