河南伏牛山发现宁陕脊蛇指名亚种
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1.河南师范大学生命科学学院,河南省太行山森林生态系统野外科学观测研究站 新乡 453007;2.济源产城融合示范区林业资源管护监测中心 济源 454600;3.河南伏牛山国家级自然保护区黑烟镇管理局 南阳 473000;4.国有南召林场 南阳 473000

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王晶晶,女,硕士研究生;研究方向:动物分类与系统进化;E-mail:wjj267224@163.com。

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中央财政国家重点野生动植物保护资助项目;


Achalinus ningshanensis ningshanensis found in Funiu Mountain of Henan, China
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1.College of Life Sciences, Henan Normal University,The Observation and Research Field Station of Taihang Mountain Forest Ecosystems of Henan Province, Xinxiang 453007; 2.Jiyuan Industry-City Integration Demonstration Zone Forestry Resources Protection, Management, and Monitoring Center, Jiyuan 454600; 3.Heiyan Town Management Bureau, Henan Funiu Mountain National Nature Reserve, Nanyang 473000; 4.State-owned Nanzhao Forest Farm, Nanyang 473000, China

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    摘要:

    本文运用形态学和分子系统学方法将采自河南省伏牛山的脊蛇样本鉴定为宁陕脊蛇指名亚种(Achalinus ningshanensis ningshanensis)。基于线粒体COI基因序列,伏牛山样本与模式产地宁陕脊蛇指名亚种存在一定的遗传分化(0.4%)。形态比较显示,伏牛山样本第2对颌片之间有2枚小鳞、腹鳞181枚、背鳞25-23-23,宁陕脊蛇指名亚种第2对颌片之间无小鳞、腹鳞161 ~ 165、背鳞为23-23-23。宁陕脊蛇指名亚种在河南省的发现,可为蛇类多样性保护和谱系地理研究提供基础资料。

    Abstract:

    Achalinus ningshanensis was first described as a new species of the genus Achalinus in 2022. It consists of A. ningshanensis ningshanensis and A. ningshanensis occidentalis. This study provides morphological and molecular information of A. n. ningshanensis collected from Funiu Mountain, Henan Province, China and determines the taxonomic status of A. n. ningshanensis in Henan.In this study, 22 morphological indexes were selected for morphological analysis. Length was measured by a vernier caliper, and the number of scales was described manually. Genomic DNA was extracted from the liver tissue preserved in 95% alcohol by the FastPure Gel DNA Extraction Mini Kit, and cytochrome c oxidase subunit I (COI) was selected as the target fragment, which was amplified with primers Chmf4 and Chmr4. COI gene sequences of 26 species of Achalinus and three outgroups (Parafimbrios lao, Fimbrios klossi, Xenodermus javanicus) were downloaded from GenBank (Appendix 1). Sequence alignment and calculation of uncorrected pairwise distances (p-distance) among congeners were performed in MEGA 7.0 software. A phylogenetic tree was constructed by maximum likelihood estimation (MLE) analysis via PhyloSuite v1.2.3 software. Morphological comparisons showed that the specimen from Funiu Mountain had two pairs of chin shields with two small scales between the second pair, 181 ventral scales, and dorsal scales of 25-23-23, while A. n.ningshanensis lacked small scales between the second pair of chin shields and had 161 ~ 165 ventral scales and dorsal scales of 23-23-23 (Table 1). The phylogenetic tree indicated that the specimen closely clustered with the topotype of A. n. ningshanensis (Ningshan, Shaanxi, China) (Fig. 3). The genetic distance between them was 0.4%, significantly smaller than the interspecific genetic distance of Achalinus (2.8% ~ 18.8%) (Appendix 2).Combining morphological and molecular phylogenetic methods, we identified the specimen of Achalinus collected from Funiu Mountain, Henan Province as A. n.ningshanensis, representing a new provincial record of snake in Henan.

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王晶晶,王爱国,廉红义,乔明奎,侯银梦,陈晓虹. 2026.河南伏牛山发现宁陕脊蛇指名亚种. 动物学杂志, 61(3): 412-423.

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  • 收稿日期:2025-04-18
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  • 在线发布日期: 2026-06-17
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