Hemiphyllodactylus is a genus characterized by high species diversity, primarily distributed across South Asia, Southeast Asia, and adjacent islands. To date, over 70 species have been described within this genus. Notably, only one species, H. jinpingensis, has been previously recorded in Guangxi Zhuang Autonomous Region, China. During a herpetofaunal survey of the Dashiwei Doline Group and Sanmenhai Scenic Cluster of the Guangxi Southwest Karst National Park conducted in Leye County, Guangxi, China, we collected two specimens belonging to this genus. The specimens were anesthetized and fixed following standard herpetological protocols. Morphological characteristics were examined and measured with a digital vernier caliper (accuracy:0.1 mm), with reference to established taxonomic keys. For molecular analysis, total genomic DNA was extracted from liver tissue, and the mitochondrial ND2 gene was amplified using primers L4437b and H5934. Phylogenetic trees were constructed using maximum likelihood (ML) and Bayesian inference (BI) methods via raxmlGUI 1.3 and MrBayes 3.2.4, respectively. MEGA 11 was used for sequence alignment, and calculation of uncorrected p-distances. Morphologically, the specimens closely matched the diagnostic features of H. yanshanensis, a species originally described from Yanshan County, Yunnan Province (Fig. 2 and Table 3). The specimens also exhibited a slightly larger maximum snout-vent length (49.1 mm) compared to the original description (46.3 mm), although all other morphological traits remained consistent. Phylogenetic trees based on ND2 sequences confirmed this identification, with the Guangxi specimens forming a strongly supported clade with the type series of H. yanshanensis (Fig.1). The uncorrected p-distance between these lineages was only 1.89% (Table 2), which is substantially lower than that between H. yanshanensis and other congeneric species (4.51% ~ 28.42%), further validating their conspecificity. This study documents H. yanshanensis for the first time in Guangxi, thereby extending its known distribution from a single locality in Yunnan Province to the northern Karst regions of Guangxi. The finding highlights the biodiversity potential of Guangxi’s Karst ecosystems and underscores the need for continued field surveys and molecular assessments. Additionally, we re-examined historical records of H. jinpingensis from Guangxi, suggesting that previous identifications may be erroneous or unverified. Further taxonomic revisions and collection efforts are necessary to clarify the taxonomic status of these populations and to improve our understanding of species diversity within this cryptic genus.