四川叙永发现中华湍蛙及其系统发育关系探讨
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西南野生动植物资源保护教育部重点实验室 南充 637009

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彭希睿,男,硕士研究生;研究方向:动物学;E-mail:13558867675@163.com。

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基金项目:

国家自然科学基金项目(No. 32270461);


The Discovery and Phylogenetic Relationship of Amolops sinensis in Xuyong, Sichuan, China
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Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong 637009, China

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    摘要:

    2023年5月及2024年6月,在位于四川省叙永县的画稿溪国家级自然保护区采集到6号湍蛙属(Amolops)标本。经过形态学和分子生物学鉴定,确认为中华湍蛙(A. sinensis),且是四川省两栖动物分布新记录种。本研究展示了该标本的16项外形特征测量值,并描述了其生境特征。对4个不同地理种群的中华湍蛙基于COI基因片段构建最大似然树,四川画稿溪标本与广东石门台模式产地标本序列聚在一起,且两者之间的遗传距离为0 ~ 0.7%,远小于同属物种之间的平均遗传距离3.1%,以及与近缘属物种滇蛙(Nidirana pleuraden)、花臭蛙(Odorrana schmackeri)、沼水蛙(Hylarana guentheri)间的平均遗传距离(8.5%)。本发现有助于理解该物种的潜在分布区以及谱系地理。

    Abstract:

    In May 2023 and June 2024, six Amolops specimens were collected in Huagaoxi National Nature Reserve, Xuyong County, Sichuan Province. Due to the large number of Amolops species and the existence of highly similar relatives, molecular identification was conducted.Morphometric measurements were taken on the collected specimens followed by extraction, sequencing, and analysis targeting mitochondrial COI gene segments derived from muscle tissue for phylogenetic inference at intra-genus level.The maximum likelihood tree of COI gene fragments from four different populations of A. sinensis showed that the Xuyong specimen from Sichuan and type locality Shimentai sequence from Guangdong were clustered together, and the genetic distance between them was 0﹣0.7%, which is significantly smaller than the average genetic distance within the Amolops species (3.1%), and the average genetic distance between them and the outgroup (closely related species Nidirana pleuraden, Odorrana schmackeri, Hylarana guentheri) of 8.5%. Meanwhile, the distribution area of A. sinensis was predicted using MaxEnt, with an average test AUC of 0.986 and a standard deviation of 0.007 after repeated runs.The species previously identified as A. ricketti, which was distributed in Sichuan Province, should be revised as A. sinensis, representing a new species record of amphibians in Sichuan Province. This study further expanded the altitude range of A. sinensis, extending the low altitude to 400 m in comparison with the previous record (500﹣1 300 m). The discovery will play an important role in understanding the potential range and phylogeography of the species.

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彭希睿,杨超,黄燕.2025.四川叙永发现中华湍蛙及其系统发育关系探讨.动物学杂志,60(2):285-294.

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  • 收稿日期:2024-07-26
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  • 在线发布日期: 2025-04-25
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