Abstract:In May 2023 and June 2024, six Amolops specimens were collected in Huagaoxi National Nature Reserve, Xuyong County, Sichuan Province. Due to the large number of Amolops species and the existence of highly similar relatives, molecular identification was conducted.Morphometric measurements were taken on the collected specimens followed by extraction, sequencing, and analysis targeting mitochondrial COI gene segments derived from muscle tissue for phylogenetic inference at intra-genus level.The maximum likelihood tree of COI gene fragments from four different populations of A. sinensis showed that the Xuyong specimen from Sichuan and type locality Shimentai sequence from Guangdong were clustered together, and the genetic distance between them was 0﹣0.7%, which is significantly smaller than the average genetic distance within the Amolops species (3.1%), and the average genetic distance between them and the outgroup (closely related species Nidirana pleuraden, Odorrana schmackeri, Hylarana guentheri) of 8.5%. Meanwhile, the distribution area of A. sinensis was predicted using MaxEnt, with an average test AUC of 0.986 and a standard deviation of 0.007 after repeated runs.The species previously identified as A. ricketti, which was distributed in Sichuan Province, should be revised as A. sinensis, representing a new species record of amphibians in Sichuan Province. This study further expanded the altitude range of A. sinensis, extending the low altitude to 400 m in comparison with the previous record (500﹣1 300 m). The discovery will play an important role in understanding the potential range and phylogeography of the species.