This study was carried out in Cheng County, Longnan City, Gansu Province in April 2023, with the aim of understanding the diversity and distribution patterns of Chiroptera species in the region and providing fundamental data for biodiversity conservation. During the survey, four bat specimens (3 ♂, 1 ♀) were collected by hand netting and the morphology and skull characteristics of these specimens were measured using electronic digital calipers. The Cyt b and COI gene sequences of three of the bat specimens (specimen numbers:GS2023023, GS2023029, and GS2023038) were sequenced and uploaded to the NCBI database (Table 1). A phylogenetic tree was constructed using the maximum likelihood method in IQ-TREE, and intraspecific and interspecific and intergeneric genetic distances for the Fanjingshan Tube-nosed Bat Murina fanjingshanensis Cyt b and COI gene were calculated based on the Kimura 2-parameter model. The following morphological measurements were taken:head and body length 47.99﹣49.51 mm, forearm length 40.15﹣42.60 mm, tibia length 16.97﹣19.30 mm, hind-foot length 9.95﹣10.53 mm (Table 2). The greatest length of the skull is 18.17﹣19.30 mm; the braincase height is 7.51﹣8.97 mm; braincase slightly swollen. Sagittal crest and lambdoid crests are not evident (Fig. 1 and Table 2). The dorsum consists of underfur and guard hairs. The base of the guard hairs is dark gray to black, the shaft is light brown, and the tips are dark brown with a reddish brown (Fig. 1). The above characteristics are consistent with the appearance and skull characteristics of M. fanjingshanensis. Phylogenetic analysis based on Cyt b and COI gene sequences showed that it was clustered with M. fanjingshanensis specimens from Guizhou (type specimen) and Hunan (Fig. 2). The genetic distance analysis showed that the intra-species genetic distance of M. fanjingshanensis was remarkably low, with a value less than 1%; the inter-species genetic distance of the same genus ranged from 2% to 22%; and the inter-generic genetic distance was 16%﹣23% (Appendices 1 and 2). The integration of external morphology, skull characteristics, and molecular phylogenetics supports the identification of M. fanjingshanensis, marking a new record of Chiroptera distribution in Gansu Province.