Abstract:With more field work and investigations being conducted, many cryptic species have been described in the genus Leptobrachella, indicating that the species diversity of Leptobrachella was underestimated. L. sungi is known from Northern Vietnam and southern Guangxi of China. In June 2020, a specimen of the genusLeptobrachella resembling L. sungi wascollectedfrom Shuitouhou Mountain, Hekou Yao Autonomous County, Yunnan Province, China (22°37′43″ N,103°52′39″ E). In this study, we examined the specimen to provide additional data on taxonomy and distribution of genus Leptobrachella. The newly collected Leptobrachella specimen from Hekou, Yunnan was identified based on morphological characters and molecular phylogenetic analyses. Morphologically, this specimen was measured and compared with type specimens of L. sungi. Measurements included:snout-vent length (SVL), head length (HL), head width (HW), snout length (SL), internarial distance (IND), interorbital distance (IOD), upper eyelid width (UEW), eye diameter (ED), nostril-eye distance (DNE), tympanum diameter (TD), forearm and hand length (FHL), tibia length (TL), foot length (FL), and length of foot and tarsus (TFL). Fragment encoding 16S rRNA was amplified and sequenced. The Kimura 2-parameter model was used to calculate the genetic distances between species of the genus Leptobrachella in MEGA 7 based on 16S rRNA gene. Subsequently, Bayesian phylogenetic analysis was conducted based on the sequences of mitochondrial 16S rRNA gene in MrBayes v.3.2.6. Morphologically, the specimen is basically consistent with type specimens of L. sungi (Figs. 2, 3, Table 2). Molecularly, the Bayesian phylogenetic tree showed that the specimen was clustered together with topotypes of L. sungi with a posterior probability of 1.00, and the genetic distance between them was 0.0% (Appendix 1, Fig. 4). The above results indicate that the specimen collected from Shuitouhou Mountain, Hekou County, Yunnan Province is L. sungi, which is a new record in Yunnan Province. This study extended the distribution area of L. sungi from north of Vietnam northward to Yunnan Province, China and provided new information for biogeography and biodiversity research.