云南河口发现三岛掌突蟾
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1.广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室,广西师范大学广西珍稀濒危动物生态学重点实验室 桂林 541004;2.海南省热带动植物生态学重点实验室,海南师范大学生命科学学院热带岛屿生态学教育部重点实验室 海口 571158

作者简介:

唐尚婧,女,本科生;研究方向:两栖类分类与进化;E-mail:sjtang163@163.com。

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基金项目:

国家自然科学基金项目(No. 32060114),广西自然科学基金项目(No. 2022GXNSFAA035526);


Leptobrachella sungi Found in Hekou, Yunnan, China
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Affiliation:

1.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin 541004; 2.Ministry of Education Key Laboratory for Ecology of Tropical Islands & Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China

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    摘要:

    2020年6月21日,在云南省河口瑶族自治县水头后山采集到1号角蟾科(Megophryidae)掌突蟾属(Leptobrachella)物种标本(标本号:GXNU YU000535)。经形态特征比较,该标本与三岛掌突蟾(L. sungi)模式标本的形态特征基本一致;基于16S rRNA基因序列构建系统发育树的结果显示,该标本与三岛掌突蟾聚为一支,且具有高支持率(贝叶斯后验概率1.00);基于Kimura双参数模型估算该标本与三岛掌突蟾的遗传距离为0.0%,与同属其他物种间遗传距离为6.8% ~ 17.9%,与外群腺角蟾(Xenophrys glandulosa)间的遗传距离为22.6%。综合形态特征比较、分子系统发育分析和遗传距离的证据,最终鉴定此次采集的标本为三岛掌突蟾,是云南省两栖动物分布新记录种。

    Abstract:

    With more field work and investigations being conducted, many cryptic species have been described in the genus Leptobrachella, indicating that the species diversity of Leptobrachella was underestimated. L. sungi is known from Northern Vietnam and southern Guangxi of China. In June 2020, a specimen of the genusLeptobrachella resembling L. sungi wascollectedfrom Shuitouhou Mountain, Hekou Yao Autonomous County, Yunnan Province, China (22°37′43″ N,103°52′39″ E). In this study, we examined the specimen to provide additional data on taxonomy and distribution of genus Leptobrachella. The newly collected Leptobrachella specimen from Hekou, Yunnan was identified based on morphological characters and molecular phylogenetic analyses. Morphologically, this specimen was measured and compared with type specimens of L. sungi. Measurements included:snout-vent length (SVL), head length (HL), head width (HW), snout length (SL), internarial distance (IND), interorbital distance (IOD), upper eyelid width (UEW), eye diameter (ED), nostril-eye distance (DNE), tympanum diameter (TD), forearm and hand length (FHL), tibia length (TL), foot length (FL), and length of foot and tarsus (TFL). Fragment encoding 16S rRNA was amplified and sequenced. The Kimura 2-parameter model was used to calculate the genetic distances between species of the genus Leptobrachella in MEGA 7 based on 16S rRNA gene. Subsequently, Bayesian phylogenetic analysis was conducted based on the sequences of mitochondrial 16S rRNA gene in MrBayes v.3.2.6. Morphologically, the specimen is basically consistent with type specimens of L. sungi (Figs. 2, 3, Table 2). Molecularly, the Bayesian phylogenetic tree showed that the specimen was clustered together with topotypes of L. sungi with a posterior probability of 1.00, and the genetic distance between them was 0.0% (Appendix 1, Fig. 4). The above results indicate that the specimen collected from Shuitouhou Mountain, Hekou County, Yunnan Province is L. sungi, which is a new record in Yunnan Province. This study extended the distribution area of L. sungi from north of Vietnam northward to Yunnan Province, China and provided new information for biogeography and biodiversity research.

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唐尚婧,谭雪婷,余国华,王剑.2025.云南河口发现三岛掌突蟾.动物学杂志,60(2):263-274.

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  • 收稿日期:2024-03-04
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  • 在线发布日期: 2025-04-25
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