Lycodon cathaya Wang, Qi, Lyu, Zeng & Wang, 2020 is a wolf snake species originally described based on specimens from Longsheng County, Guilin City, Guangxi Zhuang Autonomous Region, China. This species has subsequently been reported in Hunan Province, China. Our study aims to confirm the new specimen collected in Guizhou Province, China and to revise the diversity and distribution of Lycodon snakes in Guizhou Province. During our field survey on July 18th, 2023, one male adult specimen (CIB 119811) of the genus Lycodon was collected in Leli Town, Rongjiang County, Qiandongnan Miao and Dong Autonomous Prefecture, Guizhou Province, China (108°47′ E, 26°31′ N, 884 m a.s.l.) (Figs. 1, 2, and 5). The genomic DNA of the newly collected specimen was extracted and then amplified using primers for mitochondrial cytochrome b gene (Cyt b). We added sequences from GenBank to infer a Bayesian phylogenetic tree of the genus Lycodon (Appendix 1). The morphological data was measured using the same method from previous studies. We also reviewed literatures on the herpetofauna of Guizhou Province to summarize the diversity and distribution of Lycodon snakes within the region. The new specimen is nested within the clade of L. cathaya with strong support (Bayesian posterior probability, BPP 0.99) (Fig. 4). Morphological comparison also suggests the new specimen to be L. cathaya (Figs. 1﹣3, Appendix 2). Combining both morphological evidence and molecular phylogenetic tree inferred from mitochondrial Cyt b gene fragments, the new specimen was identified as L. cathaya, which represents a new snake record in Guizhou Province, China. Six Lycodon species are currently known from Guizhou Province with one problematic record, L. fasciatus. In addition, given the existence of the cryptic species formerly classified as L. fasciatus in China, comprehensive research on the taxonomic status of L. fasciatus from Guizhou Province is needed in future investigation.