[Objectives] Temperature is an important factor that affects the growth, development, and metabolism of aquatic organisms. Temperature tolerance significantly affects the survival and expansion of the invasive Apple Snail Pomacea canaliculata. [Methods] Transcriptome sequencing and metabolomic analysis were used to examine the alterations in the hepatopancreas of P. canaliculata exposed to high (36 ℃) and low (10 ℃) temperature stress for 48 h. After cleaning the raw sequencing data, we identified differentially expressed genes by |log2FoldChange| > 1 and P < 0.05. Gene ontology (GO) annotation and pathway enrichment analysis were then performed on the differentially expressed genes. Significantly changed metabolites were identified by variable important in projection > 1, P < 0.05, and |log2FoldChange| > 1.5. Kyoto encyclopedia of genes and genomes (KEGG) was used to search for the metabolic pathways of significantly changed metabolites. The Pearson correlation coefficient was used to analyze the correlation between differentially expressed genes and significantly changed metabolites. [Results] Compared to the control group, the high-temperature group had 446 genes and 233 metabolites with differential expression, while the low-temperature group had 288 genes and 119 metabolites exhibiting differential expression. The majority of genes and metabolites were upregulated in pathways related to amino acids, lipid transport, and immune system processes (Fig. 1), while they were downregulated in lipid acid and cytochrome P450 pathways during high-temperature stress (Fig. 2). Under low-temperature stress, significant changes were observed in alanine, aspartate, glutamate, fumaric acid, and alpha-ketoglutaric acid (Figs. 3, 4), which are involved in the energy production of the TCA cycle. Furthermore, several genes enriched in the drug metabolism pathway and heat shock proteins were found to mitigate the effects on their physiological processes. Finally, the expression patterns of these differentially expressed genes in the quantitative real-time PCR analyses were similar to those obtained by RNA-seq, indicating the accuracy and reliability of the RNA-Seq results (Fig. 6). [Conclusion] Acute temperature stress can induce immune stress and energy metabolism processes in the hepatopancreas of P. canaliculata. P. canaliculata rapidly adapts to environmental temperature changes by regulating amino acid metabolism and increasing the content of unsaturated fatty acids.