The Asian horned toad genus Boulenophrys within the family Megophryidae, is a typical representative for Oriental fauna, spreading throughout southern China, southern and eastern Himalayas, across Indochina to islands of the Sunda Shelf and the Philippines.On June 12, 2022, during the investigation of amphibian and reptile resources in Nanshan National Park of Chengbu County, Hunan Province, a female specimen (Voucher No. NS20230612) of the genus Boulenophrys, Megophryidae of Anura was collected (110°11′42″ E, 26°11′53″ N) and deposited in the Vertebrate Zoology Laboratory, College of Life Science, Hunan Normal University.The specimen was identified by morphological measurements and phylogenetic analysis based on two mitochondrial genes, namely partial 16S ribosomal RNA gene (16S rRNA) and partial cytochrome c oxidase I gene (COI). The 16S rRNA and COI gene sequences of some Boulenophrys species were also downloaded from GenBank (Table 1). According to the morphological characteristics, the back of the specimen was smooth with moles, the skin on both sides was loose, the warts were spiny, the back was grayish brown, there were dark “X” spots in the center of the back (Fig. 1). It is consistent with the morphological identification characteristics of B. mirabilis collected from Huaping Nature Reserve in Guangxi. The results of phylogenetic analysis based on 16s rRNA and COI gene fragments showed that the collected sample was clustered with B. mirabilis in one branch (Fig. 2). Combined the morphological characteristics and the reconstruction of phylogenetic relationship, the collected specimen was identified as B. mirabilis, which is a new record of amphibian in Hunan Province. The discovery of this species not only increases the species diversity of amphibians in Nanshan National Park, but also provides a reference for the taxonomy and pedigree geography of this species.