To determine the expression of gamma-aminobutyric acid B receptor(GABA_BR)gene in different tissues of Carassius auratus gibelio, GABA_BR1 gene in different tissues was amplified by RT-PCR, cloned and sequenced, its phylogenetic tree was constructed using the neighbor-joining method on the basis of homology comparison with GABA_BR1 amino acid sequences in the Gen Bank database, and additionally, the level of its expression in different tissues was further analyzed. The cDNA was 383 bp in length which encoded 127 amino acid residues.The real time PCR results showed that the GABA_BR1 gene was distributed in brain, liver, kidney, heart, intestine, muscle, bladder, gill, muscle, fin, spleen, ovary, and testis. The GABA_BR1 gene was expressed in different tissues, from high level to low level as follows: brain > caudal fin > testis > heart, intestine, bladder ovary, spleen, gill, muscle > liver, kidneys. The study confirmed the wide expression of GABA_BR1 gene in the tissues of C. auratus gibebio, with the highest expression in the brain tissue.