Short tandem repeat (STR)was amplified with fluorescence labeled primers of 17 microsatellite loci and Genetic Analyzer (ABI 3130xl)was used to separate and analyze the genotypes of the amplifiied products. The individual identification of Bulls (Bos taurus) was conducted with the standard that only one locus mismatch was permitted. The results showed that all the 17 microsatellite loci had high genetic polymorphisms, the probability of matching was 9.74×10^-10, and the cumulate discrimination power was 99.99%. This study suggests that the 17 microsatellites may be used in the bull individual identification.