A study of nest-site selection of Ardeidae birds was conducted by plot survey and line transect survey at Zhangjiang Estuary in Fujian from April to May in 2011 and 2012. The characteristics of bird nests were measured at nest site and control plots, the variable collected were used to conduct a t-test to address the habitat factors which may produce influence on nest-site selection. The study result indicated that habitat factors, including base diameter, average height, density, coverage, proportion of tree Kandelia obovata in the coverage were significantly different between nest presence plots (n=23) and control plots (n=37) (P<0.05). Also, significant differences existed highly in proportion of tree Avicennia marina, distance to road, distance to village and distance to culture pond (P<0.01). Principal component analysis shown that both of vegetation structure and disturbance sources were the main habitat factors related to nest-site selection of Ardeidae birds.Vegetation structure could influence community structure of Ardeidae birds, while as reflect the assembling breeding behavior of the birds might influence the vegetation structure.