The objective of this study is to measure the effect of supplement of fish oil, soybean oil and fish and soybean mixture oil (1∶1) in diets on the growth, immune function, metabolism and resistance to hypoxia in Chinese Mitten Crab (Eriocheir sinensis). The three diets for adult crabs with 3% fish oil, 3% soybean oil and 3% mixture oil were prepared. These diets were used for feeding crabs for 115 days. Crab weights, lengths and widths were measured. And then the crabs were placed into normal DO level (9.06±0.06 mg/L) and hypoxia water (2.57±0.44 mg/L),and their immune and metabolic indices were measured. We found that the hypoxia stress evidently influenced the metabolism of crab. The weights of crab were not significantly affected by the variation of oil lipid in diets; the hemocyte count, hemocyanin content, the activities of SOD, ALP, ACP and LDH of the crab were the highest in the mixture oil group (1 ∶1), so the supplement of fish and soybean oil (1 ∶1) in diets evidently improved the crabs’ immune and antioxidant ability, comparing to other two groups, and the crabs in mix oil group had a better ability of resistance to hypoxia.