In this study,26 microsatellite loci from the second-generation genetic linkage map of tilapia(Oreochromis spp.) were used to analyze the genetic structure of the GIFT strain Nile tilapia(O.niloticus) consisting of 60 families introduced by Freshwater Fisheries Research Center.A total of 124 alleles were detected in the population by 26 microsatellite primers,and the number of allele at each locus ranged from 3 to 7,with an average of 4.8.The average observed heterozygosity was 0.622 1 while the average expected heterozygosity was 0.642 3.The average polymorphism information content(PIC) was 0.633 4.Out of 26 detected loci,25 loci were in a high polymorphism degree(PIC > 0.5),while the remaining 1 locus was in a medium polymorphism degree.The results indicate rich polymorphism information content and large geneticdiversity in the population.This introduced GIFT strain Nile tilapia population has a large selection potential and may be used as a base population for further selection.