Many testis-specific and stage-specific genes are required for mouse spermatogenesis. In this study, semi-quantitative RT-PCR was used to confirm the tissue distribution of 12 mouse testicular genes, and fluorescence quantitative PCR labeled with SYBR GreenⅠ was applied to demonstrate the stage-specific expression of candidate genes at different spermatogenic stages. The results showed that all the genes examined were highly expressed in testis. The expression of Prm1, Prm2, Tnp1, and Tnp2 was predominant in elongating spermatids, 1.9-, 2.8-, 3.2-, and 2-fold increase as compared with that in pachytene spermatocytes, respectively. Dnajb3 expression was up-regulated 2.5-fold from pachytene spermatocytes to elongating spermatids. Akap4 was strongly expressed at elongating spermatid stage, with 5.5-fold increase than that in pachytene spermatocytes. Both Spata3 and Spata4 were equally expressed in round and elongating spermatids, 3-and 1.5-fold increase as compared with pachytene spermatocytes, respectively. The expression levels of hils1 and Tex24 were the highest in round spermatids, with 1.9-and 1.4-fold increase from pachytene spermatocytes, respectively. Spag4l and Papolb were down-regulated from pachytene spermatocytes to elongating spermatids, decreasing 45% and 34%, respectively. The results not only demonstrate the stage-specific expression of these genes, but also provide new data for further investigations into the functions of these specific genes during spermatogenesis.