During a field survey in the Second Tibetan Plateau Scientific Expedition, we collected one adult female, one male juvenile, and six tadpoles of Megophrys major (Megophryidae) group from Dulongjiang Town, Gongshan County, Yunnan Province, China. A DNA sequences data set combined partial 16S rRNA and COI genes of M. major group were analyzed (Table 1). Results show that these specimens cluster into the same lineage with M. periosa (Fig. 2), and uncorrected pairwise distance of 16S rRNA gene with types of M. periosa range from 0.004﹣0.006. Furthermore, morphological comparisons confirmed that these specimens were M. periosa, which is a new record for Yunnan Province, China. In addition, we describe the tadpole of this species. For tadpoles in Goser stage 34, body elongated, body length 37% of total length; head slightly narrower than body; oral disk large, width 1.2 times of body width, opening upfront on anterior of head; 4 rows of elongated papillae scattered on upper and lower lips, radial arranged, papillae shorter on upper lips; keratodonts absent; nares closer to pupils than tip of snout; eyes round, dorsalaterally positioned; internarial distance smaller than interpupillary distance; spiracular tube not protruding beyond body wall, positioned 57% of the distance between tip of the snout and trunk-tail junction; anal tube short, positioned under central lower tail fin base; tail well developed; tail muscle height 82% of body height, width 48% of body width; upper tail fin arise at second tail myomere; tip of tail bluntly point. Coloration mostly dull, no distinct patterns present on body in most tadpoles; tail grey brown, with small or large spots scattered mostly on tail muscle, fins and skin of ventral body semitransparent; iris copper.