Here we described a new species, Rhacophorus lishuiensis Liu, Wang and Jiang, sp. nov. (Fig. 2 and 3). Holotype: WYF11032, adult male, collected from Fengyang Forest Station (Fig. 4) in Liandu, Lishui, Zhejiang, China, by LIU Bao-Quan on 8 May 2016 (28°11′51.72″N, 119°49′2.28″E, altitude 1 100 m); allotype: WYF11035, adult female, collected on 2 April 2016; paratypes: 2♂♂ (WYF11033 and WYF11034), same date and locality as holotype; other information of allotype and paratypes same as holotype. Measurements are showing in Table 2. The holotype and allotype deposited in Herpetological Museum of Chengdu Institute of Biology, Chinese Academy of Sciences, and two paratypes deposited in Zhejiang Museum of Natural History. The new species distinguished from the congeners by a combination of the following characters: 1) small body size, snout-vent length (SVL) 34.2﹣35.8 mm in males, and 45.9 mm in female; 2) diameter of tympanum (TD) greater than half diameter of eye (ED); 3) hindlimb relatively short, tibiotarsal articulation reaching the posterior corner of eye when adpressed; 4) dorsal surface smooth, without distinct tubercles; 5) dorsal surface pure green, or scattered pale bluish green small spots; 6) throat white, iris golden yellow; 7) males with an internal single subgular vocal sac, and pale yellow nuptial pad on the base of first finger. In addition, based on 2 038 based pairs (bp) of mitochondrial 12S rRNA, tRNAval and 16S rRNA sequences, we constructed phylogenetic relationships using Bayesian inference among 41 species of the genus Rhacophours (Table 1 and Fig. 1). The new species, R. lishuiensis formed a distinct lineage, and clustered with the lineage R. dorsoviridis display relatively long branch length, with high support value (Bayesian posterior probability 1.00).