青海湖斑头雁粪便细菌分离鉴定与耐药性分析
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中国林业科学研究院森林生态环境与保护研究所;中国科学院动物研究所,中国科学院动物研究所,中国林业科学研究院森林生态环境与保护研究所,中国科学院动物研究所

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国家林业局野生动物疫源疫病项目专项;中国科学院战略生物资源科技支撑体系运行专项(CZBZX-1);“十二五”国家科技支撑计划:野生动物跨境传播重要疫病监测与防控技术研究(2013BAD12B04)


Isolation, Identification and Antimicrobial Susceptibility of Fecal Bacteria from Bar-headed Geese Anser indicus from Qinghai Lake
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Key Laboratory of Forest Protection of State Forestry Administration,National Bird Banding Center of China,The Research Institute of Forest Ecology,Environment and Protection,The Chinese Academy of Forestry;National Research Center for Wildlife Born Diseases,Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,National Research Center for Wildlife Born Diseases,Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Key Laboratory of Forest Protection of State Forestry Administration,National Bird Banding Center of China,The Research Institute of Forest Ecology,Environment and Protection,The Chinese Academy of Forestry,National Research Center for Wildlife Born Diseases,Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences

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    摘要:

    为了解斑头雁粪便中携带细菌多样性及其耐药情况,对青海湖斑头雁粪便细菌进行分离培养和生化鉴定、16S rRNA基因PCR扩增、序列分析,并进行细菌耐药性试验。结果显示,从30份斑头雁粪便中共分离到123株、10类细菌,主要有大肠埃希菌、水生拉恩氏菌、蒙氏肠球菌、枯草芽孢杆菌、柠檬节杆菌、腐败希瓦氏菌、河生肠杆菌、成团泛菌、杀鲑气单胞菌和产酸克雷伯菌。其中水生拉恩菌、杀鲑气单胞菌和成团泛菌表现为多重耐药性;其他细菌对氨苄西林和四环素有一定的耐药性,对其余受试药物都有不同程度的敏感性。表明斑头雁粪便中携带一些条件致病菌,并存在一定的耐药性,这对对野生动物疫病的监测与防控有重要意义,也为研究野鸟携带细菌的耐药机制提供理论依据。

    Abstract:

    Fecal bacteria from Anser indicus in Qinghai Lake were isolated and identified by colonial morphology observation, biochemical properties tests, amplification of 16S rRNA genes by PCR and sequences analysis. The resistance of isolates to 13 drugs were evaluated either. The results shows that 123 bacteria strains which belongs to 10 genus were isolated from 30 feces, they were Escherichia coli,Rahnella aquatilis, Enterococcus mundtii, Bacillus sublitis, Arthrobacter citreus, Shewanella pulrefaciens, Enterobacter amnigenus, Pantoea agglomerans, Aeromonas salmonicida, Klebsiella oxytoca respectively. Among these bacteria, Rahnella aquatilis, Aeromonas salmonicida and Pantoea agglomerans were multidrug resistant, and other bacteria were resistant to Ampicillin and tetracycline. The results obtained from this study indicated that Anser indicus would take amounts of pathogen which exhibit antibiotic resistance. It is of great value to the surveillance of wildlife disease, and provides references to study the antibiotic resistance mechanicsm of bacteria in wild birds.

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陈卓,罗静,张国钢,何宏轩.2016.青海湖斑头雁粪便细菌分离鉴定与耐药性分析.动物学杂志,51(4):590-598.

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  • 收稿日期:2015-11-19
  • 最后修改日期:2016-03-15
  • 录用日期:2016-04-14
  • 在线发布日期: 2016-07-19
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