To investigate the effect of Shandan Sphallerocarpus racills polysaccharide (SRP) on the urgent hepatic injury in mice (Mus musculus) induced by carbon tetrachloride (CCl4)，50 mice were gavaged with 0.2 ml SRP at different concentrations (12.5，25.0，37.5g/L) or physiological saline for 7 days，and intraperitoneally injected with 0.2 ml CCl4 solution (1% in olive oil) at the last day, the mice were sacrificed 16 h later. The morphological changes of hepatic tissues were observed under microscopy，the activities of plasma Alanine aminotransferase (ALT)，Aspartate aminotransferase (AST) were determined by colorimetry，the expression change of Transforming growth factor-β1(TGF-β1) in liver tissue were determined by immunohistochemical method．The dates were processing with spss 13.0. The results expressed in Mean ± SD. Results showed that contrast with the natural control group, the body weights are decreased in the experimental control group(Table 1), activities of plasma ALT，AST are significantly increased in the experimental control group（P < 0.01）（Table 2），the livers tissue structure are tumefaction clearly，liver cells are necrosis and seriously vacuolation in the experimental control group（Fig. 1），the positive expression of TGF-β1 in hepatic tissues are significantly increased (P< 0.01) in the experimental control group（Table 3）．However，contrast with the experimental control group, the body weights in the SRP groups are significantly increased（Table 1），the activity of plasma ALT，AST in the SRP groups are significantly decreased（P < 0.01）（Table 2），the liver have no obvious swelling，hepatic cord are clear，inflammatory necrosis are rarely，the liver celluar stucture are clear in the SRP groups（Fig. 1），the positive expression of TGF-β1 are decreased significantly（P < 0.01）in the SRP groups（Table 3）. Indicate that SRP may enhance the cellular activity，accelerate economy metabolism，and reduce cellular languish，protect the liver damage induced by carbon tetrachloride in mice．