四氯化碳联合二乙基亚硝胺对小鼠精巢的毒性作用
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渭南师范学院 化学与生命科学学院 陕西 渭南 714009,渭南师范学院 化学与生命科学学院 陕西 渭南 714009

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陕西省军民融合研究基金项目(15JMR18); 渭南师范学院特色学科建设项目(14TSXK04).


Damage of Carbon Tetrachloride Combining with Diethylnitrosamine on the Testis of Mice
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Weinan Normal University

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    摘要:

    研究四氯化碳联合二乙基亚硝胺对小鼠(Kunming mice)精巢的毒性作用。将昆明小鼠随机分为对照组与实验组。实验组每周2次腹腔注射含有15% CCl4的花生油溶液,同时自由饮用0.07‰的二乙基亚硝胺(DEN)连续诱导8周。对照组注射花生油,饮用蒸馏水。分别于第3、6、8周处死实验组及对照组小鼠各4只,对其精巢组织进行常规固定、包埋、切片、H.E染色,观察精巢的形态及精子数量的变化。与实验组比较,对照组小鼠体重明显增加,且和实验组差异显著;与对照组相比,实验组睾丸系数以及精子存活率均显著减少。对照组小鼠精巢都具有正常的生精小管结构,管腔中存在大量精子; 第3周实验组小鼠的精巢与对照组类似,生精小管较为完整,生精细胞稍有散乱;6周之后,生精小管变形,生精细胞排列疏松,精子数目减少且畸形;8周之后,生精小管残缺不全,生精细胞和精子散乱排列,精子数量大大减少且变形更为严重。实验说明,CCl4联合DEN能够造成小鼠精巢组织的损害以及精子数目的减少和畸变。

    Abstract:

    Objective: To study the toxicity effect of carbon tetrachloride (CCl4) combining with diethylnitrosamine(DEN)on testis in mice. Methods: Kunming mouse were randomly divided into two groups. The experimental group was injected by 15% CCl4 / peanut oil solution intraperitoneally two times a week for eight weeks, and drinking 0.07‰ DEN water solution freely during the treatment. The control group was injected peanut oil and drinking purified water. Both experimental group and control group, four mouse were killed at the 3, 6, 8 weeks respectively. The testicle tissue of mouse was fixed, embedded and cut conventionally, then was stained by HE stain. The changes of testicular structure and the number and shape of sperms were observed. Values were presented as Mean±SD of three independent samples. The results were analysed by two-sample equal variance Student’s t-test (one-tailed), Statistical significance was set at P<0.05. Results: The average weight significantly increased in control group compared with the experimental group (Tab.1). Testicle organ coefficient and survive rates of the sperms were significantly decreased in experimental group compared with the control group (Tab. 2, 3). The mice of control group have normal seminiferous tubule and a large number of sperm. The testis of mice in the experimental group killed in 3 weeks is similar to the control group, with completed seminiferous tubule and a small amount of the spermatogenic cells. After 6 weeks, the testis in experimental group mice changed, with disordering spermatogenic cells,decreasing and abnormal sperm. After 8 weeks, the testis of experimental group mice had a dramatic change, about all the spermatogenic cells and sperms arranged disorderly, the number of sperm decreased and deformity of sperm increased (Fig. 1). Conclusion:CCl4 combining with DEN can damage testis structure of mice and decrease the number of sperm and induce distortion of sperm.

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李亚琳,惠亮亮.2015.四氯化碳联合二乙基亚硝胺对小鼠精巢的毒性作用.动物学杂志,50(2):294-299.

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  • 收稿日期:2014-04-24
  • 最后修改日期:2015-03-10
  • 录用日期:2014-12-16
  • 在线发布日期: 2015-03-23
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