Sinibotia reevesae is an endemic fish which lives only in the upper reaches of the Yangtze River. The present study describes and characterizes the reproductive characteristic of S. reevesae in the Tuojiang River, a tributary river of the Yangtze River. 573 specimens were monthly collected from Apirl to November 2010 in Zizhong section of the Tuojiang River. Method：Standard length, body weight and gonad weight of each specimen，and eggs’ diameter of 30 females in Ⅳ stage were measured. The specimen’s ages were identified using lapillus’s abrasive disc. The gonads’ stage were divided into six stages referencing Liu Yun (1993). Statistic analysis of all data were taken using Microsoft excel 2003 and SPSS 19.0. Results: In April the testis and the ovaries are both in stage Ⅱ. From May to August, stage Ⅳ, Ⅴand Ⅵ ovaries and testis are found, and in September both ovaries and testis are degenerated to stageⅡ (Table 1). The spawing of S. reevesae population takes place from May to August, mainly in June and July, the sex can be distinguished between pectoral fin and cloaca (Fig 1). The sex-ratio of reproductive population of female vs. male is 1:1.15. 2 or 3 year-old S. reevesae reached their first sexual maturity, and the spawning stock mainly consisted of age 2, 3, 4 and 5 groups (Table 2). Standard length, weight, gonadal stage and gondosomatic index (GSI) of males at minimum maturity are 71 mm, 5.43 g, stage Ⅴ and 4.76%, and those of females minimum maturity are 76 mm, 7.80 g, stage Ⅲ and 1.46%, respectively. The distribution of eggs diameter shows one peak (Fig 2). Standard length, weight of reproductive population range from 76 mm to 120 mm and from 7.80 g to 41.60 g, respectively (Table 3).These implies that S. reevesae spawned from May to August, especially in June and July, and has a single period of spawning a year. The eggs of S. reevesae are small and sinking, and their embryonic development must be in fast flowing river.