To understand the characteristics of suitability habitat for the introduced red-eared slider turtle (Trachemys scripta elegans) in Hainan， habitat selection was studied in Qionghai section of Wanquan River. Basing on 1 741 locations from 27 individuals tracked, the ecological factors such as elevation, slope, aspect, cover type, distance to water and human disturbance distance were collected. Linear regression was used to analyze the correlation of ecological factors. Using Vanderploge and Scavia's selectivity index to assess red-eared slider turtle's habitat selection for the different habitat parameters. 3S technology (GPS, RS, GIS) was used to analyze the data obtained from field surveys and satellite images, and classifying remote sensing data of Landsat TM digital image in 2009 by supervised classification. The results show that red-eared slider turtle preferred to these area with an altitude of 0~15 m, the human disturbance distance to water from 200 to 400 m, and artificial forest distance to water below 100 m. The analysis of GIS show that the most distribution of suitable habitat in central and eastern Qionghai, and the area of suitable habitat, less suitable habitat and unsuitable habitat were 95.13 km2, 153.32 km2 and 1 432.58 km2, accounting for 5.66%, 9.12% and 85.22% respectively of the total area. It was also implied that the red-eared slider turtle has a lot of potential invasion area in Qionghai section of Wanquan River. To some extent, the analysis on the habitat suitability can provide the basis data for preventing and controlling the red-eared slider turtle in Hainan.