Vision distance and color selection of Pardosa pseudoannulata were studied by distance effect testing (3 cm, 4 cm, 5 cm, 6 cm, 7 cm) and color effect testing (redness, orange, yellowness, greenness). The testings were carried out by using P. pseudoannulata and dead Drosophila melanogaster as experiments material and two different groups of experiments equipments which were devised by ourselves. To confirm P. pseudoannulata’s vision distance, we observed and recorded their choice time of D. melanogaster in the different distances when they were hungry. Besiders, we ovbserved and recorded P. pseudoannulata’s choice time of different colors to test their color selection. Preference selection index (PSI) was used to express P. pseudoannulata’s choice situation, which meaned P. pseudoannulata’s choice time of D. melanogaster in the different distances or one color as percentage of total testing time. We tested PSI by the One-way ANOVA test. From results of the first group testing, we found out: (1) P. pseudoannulata had distance perception in vision and they had sensitivity of vision for the D. melanogaster in the distance of three, four, five and six centimeters. (2) P. pseudoannulata had the best and similar sensitivity of vision for the D. melanogaster in the distance of three amd four centimeters. However, the sensitivity of vision for the D. melanogaster in the distance of five centimeters exhibited significant decrease (P<0.05). For the D. melanogaster in the distance of five and six centimeters, P. pseudoannulata also had similar sensitivity. P. pseudoannulata’s sensitivity of vision almost defunctionalized in the distance over seven centimeters. (2) The standard curve of P. pseudoannulata’s choice time of D. melanogaster in the different distances: y=-9.6770x 118.74, R2=0.8378 and the standard curve of P. pseudoannulata’s choice time of “none D. melanogaster” in the different distances: y=9.6750x-18.729, R2=0.8377 suggested that sensitivity of P. pseudoannulata’s vision decreases correspondingly with an increase of D. melanogaster’s distance. The other group testing gave the PSI of choosing color were 35.40%±1.60% ?(redness),?36.03%±1.60% ?(greenness),?18.01%±1.60% ?(yellowness),?and?10.56%±1.60% ?(orange). Sensitivity to yellowness and orange were significantly lower than redness and greenness (P<0.05), indicating that they have the highest sensitivity to redness and greenness. It meaned P. pseudoannulat had different level sensitivity for different wavelength light.