| 宋佳,梁福轩,毛雨垚,郑普阳,谢锋,王刚.2023.四川宜宾发现黔北角蟾.动物学杂志,58(2):299-306. |
| 四川宜宾发现黔北角蟾 |
| Boulenophrys qianbeiensis Found in Yinbin, Sichuan, China |
| 投稿时间：2022-01-06 修订日期：2023-03-20 |
| DOI：DOI: 10.13859/j.cjz.202302014 |
| 中文关键词: 两栖类 布角蟾属 分布新记录种 四川省 |
| 英文关键词:Amphibian Boulenophrys New record Sichuan Province |
| 基金项目:国家自然科学基金项目（No. 32001114），成都师范学院科研创新团队立项基金项目（No. CSCXTD2020B11） |
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| 中文摘要: |
| 2021年7月在四川省宜宾市筠连县大雪山风景区乌岭沟东北（27°53′ N，104°45′ E，海拔1 380 m）采集到2号角蟾科（Megophryidae）布角蟾属（Boulenophrys）物种标本（标本号：WG202107005和WG202107006）。通过形态学比较并基于线粒体16S rRNA和细胞色素氧化酶亚基I（COI）基因序列构建的最大似然树显示，2号标本是黔北角蟾（B. qianbeiensis）。目前，已知黔北角蟾分布于贵州省桐梓县黄连自然保护区和贵州省宽阔水国家级自然保护区，此次在四川宜宾发现黔北角蟾确定为四川省两栖类分布新记录种。 |
| 英文摘要: |
| The genus Boulenophrys belongs to the family Megophryidae, Anura. This genus includes 56 species, and they are widely distributed in Sichuan, Guizhou, Zhejiang, Fujian, Jiangxi, Guangdong and other regions in China. Currently, 5 species of genus Boulenophrys have been recorded in Sichuan Province, including B. binlingensis, B. minor, B. ombrophila, B. spinata and B. wushanensis. In July 2021, two subadult amphibians were collected from Wulinggou, Daxueshan town (27°53′ N, 104°45′ E, 1380m) in the Daxueshan Scenic Area (Yibin City, Sichuan Province, China). The specimens were measured according to Fe et al.’s method (2001), and their mitochondrial 16S rRNA and COI genes were amplified and sequenced. We gathered all available 16S rRNA and COI sequences of the Boulenophrys genus and the corresponding sequences of Brachytarsophrys carinense from GenBank (Table 2), and used maximum likelihood method to build a phylogenetic tree of Boulenophrys. Genetic distances among species were measured using uncorrected p-distances calculated by MEGA 7. Through morphometric measurements (Table 1) and external morphological (Fig. 2) examination, the two specimens were found to be similar to B. qianbeiensis. Two mitochondrial 16S rRNA and COI gene sequences, length 1094 bp, were obtained. The phylogenetic tree of species in Boulenophrys showed that the two specimens were clustered with the type of B. qianbeiensis (Fig. 3). and the genetic distance of COI gene between the specimen and B. qianbeiensis was 0.000﹣0.004, whereas the genetic distance between the specimen and the B. omeimontis group was 0.038﹣0.113. Phylogenetic analysis based on 16S rRNA and COI genes as well as morphological traits have supported the two specimens were B. qianbeiensis, which should be a new amphibian record of Sichuan Province. Previously, B. qianbeiensis was only found in Huanglian Nature Reserve and Kuankuoshui National Nature Reserve in Guizhou Province. Our discovery not only expanded the distribution range of B. qianbeiensis, but also provided important information for protection and evolutionary research of B. qianbeiensis. |
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