| 吴志灵,赖洁玲,张廷瑞,白林壮,黄亮亮,郜二虎,贝永建.2022.广西发现腹斑掌突蟾.动物学杂志,57(4):620-628. |
| 广西发现腹斑掌突蟾 |
| Leptobrachella ventripunctata Found in Guangxi Province, China |
| 投稿时间：2021-11-23 修订日期：2022-07-06 |
| DOI：10.13859/j.cjz.202204016 |
| 中文关键词: 腹斑掌突蟾 广西 分布新记录种 |
| 英文关键词:Leptobrachella ventripunctata Guangxi New record |
| 基金项目:国家林业和草原局全国第二次陆生野生动物资源调查项目“南盘江流域中山峡谷-广西（Oc01-1）野生动物资源调查” |
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| 中文摘要: |
| 2021年5月在广西隆林县者浪乡坡合村（105°13′27″ E，24°44′58″ N）采集到5号两栖动物标本，经形态特征比较，与腹斑掌突蟾（Leptobrachella ventripunctata）相似；基于线粒体16S rRNA和12S rRNA基因片段构建的贝叶斯系统发育树显示，此次采集到的掌突蟾属标本与腹斑掌突蟾聚为一支，且具有较高的后验概率（1.00）；基于Kimura双参数模型估算本次采集的标本与腹斑掌突蟾的遗传距离为0.7% ~ 0.9%，远小于掌突蟾属物种间的遗传距离（4.4% ~ 23.4%）。综合形态特征和系统发育分析比较，鉴定此次采集的掌突蟾属标本为腹斑掌突蟾，为广西两栖动物分布新记录种。 |
| 英文摘要: |
| Five specimens (2 males and 3 females) of the Leptobrachella were collected in Pohe Village, Zhelang Township, Longlin County, Guangxi, China at 105°13′27″ E, 24°44′58″ N altitude 826 m during a field survey in May 2021. The specimens were measured with digital calipers for following measurements: snout-vent length, head length, head width, snout length, internasal space, interorbital space, width of upper eyelid, diameter of eye, diameter of tympanum, length of lower arm, hindlimb length, tibia length, foot length, and compared these characters with the L. ventripunctata. All specimens were fixed in 10% buffered formalin, transferred to 95% ethanol, and deposited in Yulin Normal University. Five liver samples attained from euthanasia specimens and then preserved in 95% ethanol and stored at ﹣20 ℃ were used for molecular analysis. All samples were 17 sequences from all known Leptobrachella species, two sequences from the out-group Brachytarsophrys and Leptobrachium were obtained from GenBank and incorporated into our dataset (Detail information of these materials was shown in Table 2). Genomic DNA was extracted from liver tissue samples using DNA extraction kit. Partial sequences of 12S rRNA and 16S rRNA gene were amplified according the method of Lyu et al. (2017). The resulting fragments were sequenced with both forward and reverse primers. The Clustal W algorithm was used in MEGA 7.0 software to compare all the 12S rRNA and 16S rRNA gene sequences and the Kimura two-parameter model was used to calculate the genetic distances between species of the genus Leptobrachella. We assessed phylogenetic and systematic relationships among the species mentioned above of the mitochondrial DNA genes of 12S rRNA and 16S rRNA using maximum likelihood and Bayesian inference methods by PhyloSuit v1.2.2 software. In this study, we found that the specimens were similar with L. ventripunctata in morphological, morphometric measurements (Table 3) and feature (Fig. 1). Bayesian inference tree and maximum likelihood tree showed that the five specimens were clustered with the L. ventripunctata with a high support, which the Bayesian posterior probabilities (PP) were 1.00 and the bootstrap support (BS) was 100 (Fig. 2). The genetic distance between the specimens and L. ventripunctata was 0.7%﹣0.9%, much lower than that between Leptobrachella species (4.4%﹣23.4%) (Table 3). The specimens from Longlin County are identified as L. ventripunctata based on morphological and molecular comparison, and it is a new record of this species in Guangxi, China. |
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