| 刁迎珠,郭飞燕,李雅琦,陈柏达,裴鑫怡,王萍,彭秀娟,李文慧,张志强.2022.聚苯乙烯暴露和清除对黑斑侧褶蛙蝌蚪身体大小和脏器系数的影响.动物学杂志,57(3):429-439. |
| 聚苯乙烯暴露和清除对黑斑侧褶蛙蝌蚪身体大小和脏器系数的影响 |
| Effects of Fluorescent Polysterene Microspheres on Body Size and Internal Organ Indices in the Tadpoles of Pelophylax nigromaculatus |
| 投稿时间：2021-09-22 修订日期：2022-04-07 |
| DOI：10.13859/j.cjz.202203012 |
| 中文关键词: 黑斑侧褶蛙 聚苯乙烯 身体大小 脏器系数 蝌蚪 |
| 英文关键词:Pelophylax nigromaculatus Polystyrene Body size Internal organ index Tadpole |
| 基金项目:安徽农业大学2020年度省级重点教学研究项目（No. 2020jyxm0531），2020年度省级教学示范课项目（No. 2020SJJXSFK09），安徽农业大学2020年校级（No. XJDC2020578，No. XJDC2020114）、国家级大学生创新创业训练计划项目创新训练项目（No. 202010364075）、2021年度研究生创新基金项目（No. 2021yjs-17） |
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| 中文摘要: |
| 探究不同粒径（50 nm、200 nm和1 000 nm）、浓度（清水组、低浓度组、中浓度组和高浓度组）荧光聚苯乙烯微球溶液组合对37期黑斑侧褶蛙（Pelophylax nigromaculatus）蝌蚪身体大小和脏器系数的影响，连续测定了暴露7 d时，之后清水清除饲养7 d（14 d时）和14 d（21 d时），其体重与体全长的比值（重长比）、小肠长度以及心、肝湿重的变化。重长比在200 nm粒径条件下不受聚苯乙烯微球暴露和清除影响（P > 0.05）；50 nm清水组14 d时低于其他浓度组（P < 0.05）；1 000 nm中浓度组7 d时和14 d时高于21 d时（P < 0.05），7 d时高浓度组低于其他浓度组（P < 0.05），21 d时清水组和低浓度组都高于中浓度组（P < 0.05）。小肠长度系数在200 nm高浓度组随时间而变化，7 d时至14 d时显著增加，21 d时陡降（P < 0.05），1 000 nm中浓度组7 d时和14 d时都显著高于21 d时（P < 0.05）；7 d时，1 000 nm高浓度组显著低于其他浓度组（P < 0.05），50 nm、200 nm均无组间差异（P > 0.05）。心、肝湿重系数均不随处理时间而变化（P > 0.05）；心湿重系数只在50 nm 14 d时变化显著，清水组最高，高、中浓度组其次，低浓度组最低（P < 0.05），肝湿重系数只在50 nm和200 nm 14 d时清水组低于其他浓度组（P < 0.05）。50 nm、200 nm和1 000 nm处理分别影响重长比、心和肝湿重系数，小肠长度系数和肝湿重系数，以及重长比和小肠长度系数，但与粒径和浓度均不呈线性关系。 |
| 英文摘要: |
| [Objectives] Exploring the effects of different particle sizes (50, 200 and 1 000 nm) and different concentrations (zero, low, moderate and high) of fluorescent polystyrene microsphere solutions on the body size and internal organ indices of Pelophylax nigromaculatus at Gosner stage 37 tadpoles. [Methods] The Gosner stage 37 tadpoles were exposed to any combination of any particle size and any concentration solution for 7 days, then were transfered into dechlorinated tap water and fed without fluorescent polystyrene microsphere solution for an additional 7 or 14 days. The variations of ratio of body mass to total body length (ratio of BM to TBL), the length of small intestine, and the wet masses of heart and liver were continuously measured. All statistical analyses were performed using SPSS software packages (SPSS 19.0 for windows). For any particle size (50, 200 or 1 000 nm), two-way ANOVA (concentration × day) and multiple comparisons were used to compare the differences of ratio of BM to TBL, the length index of small intestine, and the wet mass indexes of heart and liver. Then, one-way ANOVA and multiple comparisons were further used to compare the differences of the parameters mentioned above among different concentration groups from the same day, or among different days from the same concentration group. Results were presented as means ± standard error, and P < 0.05 was considered to be statistically significant. [Results] Ratio of BM to TBL was not affected by polystyrene microsphere exposure and clearance under the conditions of 200-nm solution (Table 3, P > 0.05); The BM to TBL ratio was significantly lower in clean water group than that of any other concentration group on day 14 when exposed to 50-nm solution (Table 2, P > 0.05); When exposed to 1000-nm solution, the ratio was significantly higher on days 7 and 14 than on day 21 for moderate concentration group, which was lower in high concentration group than that of any other concentration group on day 7, and was higher in clean water group and low concentration group than that of moderate concentration group on day 21 (Table 4, P < 0.05). When exposed to 200-nm solution with high concentration, small intestine length index was significantly changed with days, being greatly increased from day 7 to day 14, and sharply declined by day 21 (Table 3, P < 0.05); On day 7 or 14, exposure to 1 000-nm solution with moderate concentration, the index was greatly higher than on day 21, while it was greatly lower in high concentration group than that of any other concentration group (Table 4, P < 0.05), but not for 50-nm (Table 2, P < 0.05) or 200-nm solution group (Table 3, P < 0.05). The wet mass indexes of heart and liver did not greatly change (Table 2﹣4, P > 0.05); The heart wet mass index was only significantly changed under the conditions of 50-nm solution on day 14, which was highest in clear water group, followed by high and moderate concentration groups, and lowest in low concentration group (Table 2, P < 0.05); The liver wet mass index was only significantly lower in clean water group than that of any other concentration group on day 14 from 50-nm or 200-nm solution group (Table 2 and 3, P < 0.05). [Conclusion] Ratio of BM to TBL, indexes of heart and liver wet masses, small intestine length index combined with liver wet mass index, and ratio of BM to TBL combined with small intestine length index were affected by 50-nm, 200-nm or 1000-nm polystyrene microspheres solution, respectively, but polystyrene particle size and concentration are not linear with their potential hazards. |
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