[Objectives] Four species of amphibian were recorded in Wanfoshan Town, Tongdao County, Hunan Province, China at 26°11′47″ N, 109°56′21″ E, altitude 924 m during a filed survey on 23 May, 2020. They are recognized as first recorded in Hunan province by morphological and molecular. [Methods] Compared the specimens with other species of the genus Nidirana morphologically by comparing their measurement data and diagnosis feature (Sample information is shown in Table 1). Molecularly, based on 16S rRNA gene constructed Bayesian inference tree to analyze its phylogenetic relationship, the Kamura two- parameter model in MEGA v6.0 was used to calculate the genetic distances between species of the genus Nidirana. [Results] Through this research, we found the specimens were similar with N. leishanensis in morphological, morphometric measurements (Table 2) and feature (Fig. 1) conform to diagnosis characters. Based on 16S rRNA gene constructed Bayesian inference tree showed that the four specimens were clustered with the N. leishanensis with a high support (1.00) (Fig. 2). The genetic distance between the specimens and N. leishanensis from type locality was 0.8%, much lower than that between Nidirana species range from 1.4% to 6.4% (Table 3). [Conclusion] Based on morphological and molecular compared, the specimens were identified as N. leishanensis, which is the new record of this species in Hunan Province, China.