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李栋梁,孙越,徐发荣.2021.乌梢蛇粪便微生物群组成和特征的 种群间差异.动物学杂志,56(5):696-706.
乌梢蛇粪便微生物群组成和特征的 种群间差异
Differences in Faeces Microbiome Composition and Characteristics Between Two Populations of Ptyas dhumnades
投稿时间:2021-02-05  修订日期:2021-07-06
DOI:10.13859/j.cjz.202105007
中文关键词:  乌梢蛇  粪便微生物群  基因功能预测
英文关键词:Ptyas dhumnades  Faeces microbiome  Gene function prediction
基金项目:
作者单位E-mail
李栋梁 北京市动物疫病预防控制中心 北京 102629 ldlldl@163.com 
孙越 北京市畜牧业环境监测站 北京 102200 422378081@qq.com 
徐发荣 北京市动物疫病预防控制中心 北京 102629 xufr001@sina.com 
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中文摘要:
      肠道微生物能够协助寄主维持体内稳态、增强营养吸收和能量代谢,对寄主适应环境和维持生存具有重要的意义。本研究以乌梢蛇(Ptyas dhumnades)桂林(n = 3)和襄阳(n = 3)种群的为研究对象,收集动物的粪便提取微生物总DNA,进行16S rRNA基因V1 ~ V3区域扩增子测序,分析两个种群乌梢蛇粪便微生物群组成、丰度和基因功能预测上的差异。结果表明,两种群的粪便微生物在a-多样性上无显著差异,两种群相对丰度较高的粪便微生物和基因功能类型存在显著差异。在门水平上,乌梢蛇粪便微生物群优势门为拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和梭杆菌门(Fusobacteria)。线性判别分析分析显示,桂林和襄阳种群的乌梢蛇粪便微生物具有显著的丰度差异特征。此外,对两种群粪便微生物基因功能预测,在与环境信息处理、代谢相关和遗传信息处理有关的基因均有不同程度的差异。未来需要更多的研究关注野外动物粪便微生物菌种组成及其与寄主的进化关系,为野生动物保护提供粪便微生物方面的相关建议。
英文摘要:
      Gut microbiota can help hosts maintain homeostasis, enhance nutrient absorption and energy metabolism, which is of great significance for the survival and environment adaption. Many factors can affect the gut microbiota, including microhabitat, captivity, seasonal changes, feeding items, sex, and development stage. In this study, the Big-eyed Ratsnake (Ptyas dhumnades) from Guilin and Xiangyang populations were selected as the research objects. The total microbial DNA was extracted from snake feces to sequence the V1﹣V3 regions of 16S rRNA gene using the amplicon sequencing and the differences in gut microbial composition, abundance, and gene function between the two populations were analyzed. At the phylum level, the dominant phyla included Bacteroidetes, Proteobacteria, Firmicutes and Fusobacteria (Fig 1). Linear discriminant analysis effect size (LefSe) analysis showed richly distributed bacteria and gene functions were different in the two populations (Fig. 2). However, there was no significant differences in a- diversity between the two populations (Fig. 3). The relatively high bacterial abundance in the Guilin population included the order Cardiobacteriales, the family Wohlfahrtiimonadaceae and the genus Koukoulia in Proteobacteria, and the members of the order Acidaminococcales and the genus Phascolarctobacterium in Firmicutes. However, the Xiangyang population owned the relatively high proportion in the order Burkholderiales, the family Aquaspirillaceaeke and the genus Microvirgula of Proteobacteria. In addition, the gene functional differences of gut microbiota between two populations were reflected in the high expression related to environmental information processing including ko02010 and ko02060 in Guilin populations. In contrast, those related to the ko02020 and ko00910 were mainly expressed in the Xiangyang population (Fig. 4). The high expression genes were related to the metabolism including ko00010, ko00520, ko00230 and ko00240 in the Guilin population, and ko00680 and ko00900 in the Xiangyang population (Fig. 4). Guilin population expressed a highly of ko03018, ko03010 and ko00970, while Xiangyang population expressed highly of ko02030. In the future, more studies are needed to focus on the composition of gut microbiota and the evolutionary relationships between gut microbiota and their hosts to provide relevant suggestions for the protection of wild animals.
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