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陈润,王亚冰,曾姣,王翠华,王倩,王建钢,施兆鸿,马凌波,彭士明.2021.低氧-酸化胁迫对大黄鱼早期发育 阶段生长及生理代谢的影响.动物学杂志,56(5):716-728.
低氧-酸化胁迫对大黄鱼早期发育 阶段生长及生理代谢的影响
Effects of Growth and Physiological Metabolism of Larimichthys crocea in Early Developmental Stages Under Hypoxia-Acidification Stress
投稿时间:2020-12-31  修订日期:2021-07-12
DOI:10.13859/j.cjz.202105009
中文关键词:  大黄鱼  低氧  酸化  生长发育  代谢
英文关键词:Larimichthys crocea  Hypoxia  Acidification  Growth development  Metabolism
基金项目:国家重点研发计划项目(No. 2018YFD0900603),现代农业(海水鱼)产业技术体系专项资金项目(No. CARS-47-G25),中国水产科学研究院基本科研业务费(No. 2020XT10)
作者单位E-mail
陈润 中国水产科学研究院东海水产研究所 上海 200090上海海洋大学水产与生命学院 上海 201306 shenban00015@163.com 
王亚冰 中国水产科学研究院东海水产研究所 上海 200090 wangyabing@ecsf.ac.cn 
曾姣 中国水产科学研究院东海水产研究所 上海 200090上海海洋大学水产与生命学院 上海 201306 ZengJiao0814@163.com 
王翠华 中国水产科学研究院东海水产研究所 上海 200090 wangch@ecsf.ac.cn 
王倩 中国水产科学研究院东海水产研究所 上海 200090 wangqianshou@126.com 
王建钢 中国水产科学研究院东海水产研究所 上海 200090 379618550@qq.com 
施兆鸿 中国水产科学研究院东海水产研究所 上海 200090 shizh@ecsf.ac.cn 
马凌波 中国水产科学研究院东海水产研究所 上海 200090 malingbo@vip.sina.com 
彭士明 中国水产科学研究院东海水产研究所 上海 200090 shiming.peng@163.com 
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中文摘要:
      为探究低氧-酸化胁迫对大黄鱼(Larimichthys crocea)早期发育阶段生长及生理代谢的影响,实验设置4个处理组,分别为对照组(溶解氧7.0 mg/L,pH 8.1)、低氧组(溶解氧3.5 mg/L,pH 8.1)、酸化组(溶解氧7.0 mg/L,pH 7.3)和低氧-酸化组(溶解氧3.5 mg/L,pH 7.3)。每个处理组4个重复,每个重复放置大黄鱼受精卵4.0 × 104粒。在实验开始(记录为0 d)和受精卵孵化后1 d、3 d、5 d、10 d、15 d、20 d、27 d测定其类胰岛素生长因子-1(IGF-Ⅰ)和生长激素(GH)含量,以及丙酮酸激酶(PK)、谷丙转氨酶(GPT)、碱性磷酸酶(AKP)和钠钾ATP酶(NKA)活性。并测定27 d时的体长和体高。实验结果表明,低氧和酸化胁迫在27 d均显著抑制大黄鱼体长、体高的增长,低氧-酸化双重胁迫的抑制效果更为明显。类胰岛素生长因子含量分别在3个处理组的多个时段显著低于对照组(P < 0.05),其中,3个处理组类胰岛素生长因子含量在3 d时均显著低于对照组(P < 0.05)。生长激素含量在不同处理组中均出现升高的趋势,27 d时3个处理组均高于对照组(P < 0.05)。丙酮酸激酶活性在低氧-酸化组1 ~ 5 d时显著高于对照组(P < 0.05),其他三个组的变化基本上呈现出先升后降的趋势。谷丙转氨酶活性在3个处理组中的多个时间点显著高于对照组(P < 0.05)。3个处理组中碱性磷酸酶活性在3 d时显著低于对照组(P < 0.05)、15 d时显著高于对照组(P < 0.05)。3个处理组钠钾ATP酶活性在3 d时均显著低于对照组(P < 0.05)。综合分析得出,在本实验条件下大黄鱼早期发育阶段在低氧和酸化胁迫的环境中个体生长均会受到抑制,低氧-酸化的双重胁迫对其抑制作用更显著。各处理组的代谢酶活性在多个时段与对照组相比发生显著性变化,结合本实验中大黄鱼个体生长差异和代谢酶活性差异的分析得出,低氧和酸化胁迫导致大黄鱼主要代谢酶活性产生了响应性的变化,进而最终影响了大黄鱼的个体生长。
英文摘要:
      In order to explore the effects of hypoxia-acidification stress on the growth and physiological metabolism of Larimichthys crocea in the early development stages, 4 treatment groups were designed, namely the control group [dissolved oxygen (DO) = 7.0 mg/L, pH 8.1], the hypoxia group (DO = 3.5 mg/L, pH 8.1), acidification group (DO = 7.0 mg/L, pH 7.3), and hypoxia-acidification group (DO=3.5mg/L, pH 7.3). Four replicates were conducted in each treatment group, and 4.0 × 104 fertilized eggs were placed in each repetition.The contents of insulin like growth factor 1 (IGF-Ⅰ) and growth hormone (GH), and the activities of pyruvate kinase (PK), glutamic pyruvic transaminase (GPT), alkaline phosphatase (AKP) and Na+-K+-ATPase (NKA) were determined after the treatment (recorded as the 0th d) and the 1st d, 3rd d, 5th d, 10th d, 15th d, 20th d and 27th d after hatching. Lengths and heights of bodies in each group on 27th d were also determined. Both hypoxia and acidification stress significantly inhibited the growth of body length and height on the 27th d, and the inhibitory effect of hypoxia-acidification stress was more significant. The contents of IGF-I were significantly lower in the three treatment groups at many time points than in the control group (P < 0.05), and the contents of IGF-I in the three treatment groups were significantly lower than in the control group on the 3rd d (P < 0.05). The contents of GH showed an increasing trend in different treatment groups, and were higher than in the control group on the 27th d (P < 0.05). The activity of PK of the hypoxia-acidification group was significantly higher than that in the control group (P < 0.05) from the 1st to the 5th d. The changes in the other three groups basically showed a trend of increasing first and then decreasing. The activities of GPT in the 3 treatment groups were significantly higher than in the control group at many time points (P < 0.05). The activities of AKP of the 3 treatment groups were significantly lower than in the control group on the 3rd d (P < 0.05), and were significantly higher than in the control group on the 15th d (P < 0.05). The activities of NKA in the 3 treatment groups were significantly lower thanin the control group on the 3rd d (P < 0.05). Conclusion: Under the experimental conditions, the individual growth of L. crocea in the early stage in the environment of hypoxia and acidification stressis inhibited, and the dual stress of hypoxia and acidification has a more significant effect. Compared with the control group, the activities of metabolic enzymes of each treatment group changesignificantlyat many time points. Combining the analysis of the individual growth differences and metabolic enzyme activities of L. crocea in this experiment, it is concluded that hypoxia and acidification stress cause responsive changes in the activities of the main metabolism enzymes , which ultimately affects the individual growth of L. crocea.
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