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靳玮江,张天祥,张美善,韩香雨,蒋元琳,张宝峰,李大卫,刘树强,胡德夫.2021.睾丸切除对麝鼠香腺形态和 麝鼠香成分的影响.动物学杂志,56(4):528-590.
睾丸切除对麝鼠香腺形态和 麝鼠香成分的影响
Changes in Scent Gland Morphology and Musk Compositions after Castration by Testicular Resection in the Muskrat
投稿时间:2020-12-14  修订日期:2021-06-17
DOI:10.13859/j.cjz.202104010
中文关键词:  麝鼠  去势  香腺  麝鼠香
英文关键词:Muskrat, Ondatra zibethicus  Castration  Scent glands  Muskrat musk
基金项目:北京市自然科学基金项目(No. 5202016)
作者单位E-mail
靳玮江 北京林业大学生态与自然保护学院 北京 100083 weijiangjin135@126.com 
张天祥 北京林业大学生态与自然保护学院 北京 100083 toughtom@163.com 
张美善 北京林业大学生态与自然保护学院 北京 100083 sddcezms@163.com 
韩香雨 北京林业大学生态与自然保护学院 北京 100083 han_xiangyuwy@163.com 
蒋元琳 北京林业大学生态与自然保护学院 北京 100083 yuanlinjiang0722@163.com 
张宝峰 北京林业大学生态与自然保护学院 北京 100083 baofengdaisy@126.com 
李大卫 北京林业大学生态与自然保护学院 北京 100083 david838656186@163.com 
刘树强 北京林业大学生态与自然保护学院 北京 100083 liushuqiang@bjfu.edu.cn 
胡德夫 北京林业大学生态与自然保护学院 北京 100083 hudf@bjfu.edu.cn 
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中文摘要:
      对麝鼠(Ondatra zibethicus)去势,探讨睾丸切除对麝鼠香腺形态和麝鼠香成分的影响。6只成年雄性麝鼠,随机分为去势组和对照组,每组3只。麝鼠香腺内香液排尽后,施行去势手术。去势30 d后,麻醉处死动物,采集和分离麝鼠的血清备用,然后采集麝鼠香。采用酶联免疫吸附法(ELISA)检测血清和麝鼠香中的睾酮(T)含量。取香腺,测量其长度,H.E染色观察香腺组织结构的变化,并测量腺细胞直径。同时,利用气相色谱-质谱联用技术(GC-MS)测定麝鼠香化学成分。去势组的血清中睾酮含量极显著低于对照组(t = 5.270,P < 0.01),去势组麝鼠香中睾酮含量显著低于对照组(t = 3.229,P < 0.05)。形态学上,去势组和对照组的麝鼠香腺长度无显著差异(t = 1.243,P > 0.05)。组织学上,去势组腺细胞直径显著缩小(t = 9.434,P < 0.01)。麝鼠香中,去势组和对照组含量最高的3类成分相同,均为大环类、类固醇和脂肪酸。其中,去势组的大环类物质含量显著升高(t =﹣3.084,P < 0.05),而类固醇含量极显著降低(t = 3.407,P < 0.05)。完成建立麝鼠去势模型,切除睾丸后导致麝鼠香腺腺细胞形态和麝鼠香成分发生了变化。
英文摘要:
      Castration surgery was accomplished on muskrats (Ondatra zibethicus) to observe the changes in scent gland morphology and musk compositions after castration. Six adult male muskrats were randomly divided into castrated and control groups (3 in each group). The musk in scent glands was drained, and then both testes were removed under general anesthesia. Thirty days after the castration, the muskrats were anesthetized and killed, and the serum and the musk were collected. The testosterone (T) concentration in serum and muskrat musk was detected by enzyme-linked immunosorbent assay (ELISA). The scent glands were collected, and their lengths were measured. The paraffin sections of scent glands were stained by the H.E method, and the diameters of glandular cells were measured. And then, the compositions of muskrat musk were detected by gas chromatography-mass spectrometry (GC-MS). The comparison of differences between the two groups was analyzed with independent-samples T test method using SPSS 20.0. The concentration of testosterone in the serum of the castrated group was significantly lower than that of the control group (t = 5.270, P < 0.01), and the testosterone content of muskrat musk in the castrated group was also significantly lower than that of the control group (t = 3.229, P < 0.05) (Table 1). Morphologically, the lengths of muskrat scent glands in the castrated group and the control group were similar and there was no significant difference (t = 1.243, P > 0.05). Histologically, the Diameter of glandular cells of the castrated group was significantly reduced compared with the control group (t = 9.434, P < 0.01) (Fig. 2). The GC-MS analysis showed that both groups contained three rich compositions: macrocyclics, steroids and fatty acids. Among them, the content of macrocyclics in the castrated group was significantly higher (t =﹣3.084, P < 0.05), while the content of steroids was significantly reduced (t = 3.407, P < 0.05) (Table 2). Thus, the muskrat castration model was established, in which the morphology of the muskrat scent gland cells and the compositions of muskrat musk changed.
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