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赵耀,聂子涵,武晓宇,孙铠,刘志平.2021.繁殖期雌性蓝狐性激素检测与 性器官组织学观察.动物学杂志,56(3):384-392.
繁殖期雌性蓝狐性激素检测与 性器官组织学观察
Sex Hormone Detection and Histological Observation of Sex Organs in Breeding Female Blue Foxes
投稿时间:2020-10-10  修订日期:2021-03-13
DOI:10.13859/j.cjz.202103008
中文关键词:  蓝狐  发情期  切片  雌二醇  孕酮  子宫  卵巢
英文关键词:Blue Fox, Alopex lagopus  Oestrum  Section  Estradiol  Progesterone  Uterus  Ovary
基金项目:国家重点研发计划项目(No. 2018YFDO502201-10),广西自然科学基金项目(No. 2018GXNSFAA294066)
作者单位E-mail
赵耀 东北林业大学野生动物与自然保护地学院 哈尔滨 150040 422340146@qq.com 
聂子涵 东北林业大学野生动物与自然保护地学院 哈尔滨 150040 591208629@qq.com 
武晓宇 东北林业大学野生动物与自然保护地学院 哈尔滨 150040 740911824@qq.com 
孙铠 东北林业大学野生动物与自然保护地学院 哈尔滨 150040 422340146@qq.com 
刘志平 东北林业大学野生动物与自然保护地学院 哈尔滨 150040 852108389@qq.com 
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中文摘要:
      为了研究繁殖期雌性蓝狐(Alopex lagopus)类固醇激素含量、发情表现与性器官组织学结构之间的关系,于2019年选取繁殖期不同发情状况的雌性蓝狐,共18只,分为典型发情、非典型发情和不发情3组。通过无损伤取样法采集粪便与尿液并通过放射免疫法(RIA)测定孕酮(P)和雌二醇(E2)的含量;每组选取1只在发情并输精的当天取卵巢和子宫,进行组织学观察。结果显示,所测定典型发情、非典型发情、不发情蓝狐类固醇激素水平,雌二醇在尿液中的含量显著高于粪便,且在不同组间差异明显,尤其在典型发情组更高,达到(11 065.17 ± 546.76)ng/L;孕酮含量尿液与粪样差异不大,孕酮含量在典型发情雌性蓝狐粪便与尿液含量差异不显著,但在不发情雌性蓝狐中差异显著,为(16.61 ±0.63)μg/L。粪尿测定孕酮和雌二醇激素含量与三组不同情况的发情表现相比相关性显著。粪、尿作为类固醇激素检测样本,虽然含量有差异,但变化趋势一致,都可使用。卵巢与子宫在典型发情雌性蓝狐中体积较大,卵巢可见各级卵泡和多个黄体,子宫黏膜上皮为柱状上皮,排列紧密,固有层内可见大量腺体;非典型发情雌性蓝狐卵巢、子宫发育状况与典型发情雌性蓝狐相类似;不发情雌性蓝狐卵巢、子宫呈静息状态,卵巢中卵泡多处于闭锁状态,无卵母细胞,也无黄体,固有层间质细胞及肌层肌细胞排列更为紧密。说明检测孕酮和雌二醇激素的含量,可以准确判定蓝狐发情的状态。
英文摘要:
      To investigate the relationship among steroid hormones, estrous behaviors, and histological changes of sexual organs in breeding female blue foxes (Alopex lagopus), female individuals in different breeding stages were designated as three groups: typical estrus, atypical estrus, and non-estrus. Estrus identification, sample collection and hormone determination were performed. The hormone level was determined by radioimmunoassay (RIA) and XH6080 radioimmunoassay system was used in Xi'an Nuclear Instrument Factory. After the standard substance and samples were fully shaken with labeled antibody, they were then placed at room temperature for 15 min, centrifuged at 3 500 r/min for 15 min, and finally the supernatant was absorbed and discarded and the radioactivity count (CPM) of each precipitation tube was measured. Urine was collected and sent directly for hormone examination, and the contents of progesterone (P) and estradiol (E2) were determined. The ovaries and uterus were taken on the day of estrus and insemination. After dehydration, paraffin embedding, serial section and H.E staining, histological detection was conducted. Observation was conducted under optical microscope at 10 × 10 times lens and 10 × 100 times lens. The results showed that the female blue fox was in different phases of estrus, including vulva, vaginal resistance and keratinocyte stages, as shown in Fig. 1﹣3. The content of estradiol in the urine was significantly higher than that in the feces, especially in the typical estrus group, reaching 11 065.17 ± 546.76 ng/L, and the difference was significant. However, the content of progesterone in the urine was not significantly different from that in the feces, as shown in Table 2. The content of progesterone in the feces was slightly higher than that of urine in typical estrus females, but significantly higher than that of the urine in non-estrus females 16.61 ± 0.63 g/L, as shown in Table 1. Progesterone and estradiol contents were significantly correlated with estrus performance. When feces and urine were used as steroid test samples, although the hormone contents were different, the hormone changing trend was the same in these two kinds of samples. The ovary and uterus were relatively larger in female blue fox in typical estrus. Follicles at different stages and multiple corpus lutea could be observed in the ovary. The epithelial cells of uterine mucosa were columnar in shape and closely arranged, and a large number of glands could be observed in the lamina propria, as shown in Fig. 4 and 5. The ovarian and uterine development of the atypical estrus female was similar to that of the typical estrus female, as shown in Fig. 6 and 7. In the ovary and uterus of non-estrus female blue fox, the follicles in the ovary were mostly in an atresia state, without oocytes or corpus luteum. The lamina propria interstitial cells and myotrophic myocytes were arranged more closely, as shown in Fig. 8 and 9. It indicates that the blue fox's estrus state can be accurately determined by detecting the content of progesterone and estradiol hormones.
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