| 唐胜,卢想,朱颖,董学颖,唐俊,刘秋宁,王刚,唐伯平.2023.基于转录组探讨中国圆田螺在干旱胁迫下休眠特征.动物学杂志,58(6):937-949. |
| 基于转录组探讨中国圆田螺在干旱胁迫下休眠特征 |
| Transcriptome Analysis of the Dormancy Characteristic Mechanism of Cipangopaludina chinensis Under Drought Stress |
| 投稿时间：2023-01-11 |
| DOI：10.13859/j.cjz.202323007 |
| 中文关键词: 中国圆田螺 转录组 休眠 差异表达基因 干旱胁迫 |
| 英文关键词:Cipangopaludina chinensis Transcriptome Dormancy Different expressed genes Drought stress |
| 基金项目:国家自然科学基金项目(No. 32270487)，江苏省科学技术厅重点研发计划项目(No. BE2020673)； |
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| 中文摘要: |
| 中国圆田螺(Cipangopaludina chinensis)属于淡水大型螺类，适应性强，分布广泛，在极端环境下能够迅速进入休眠状态，以抵御不良环境对自身造成的影响。为研究其在干旱胁迫下的休眠特征，应用高通量测序技术对中国圆田螺在干旱胁迫下肝与肾组织进行转录组测序及分析。结果显示，中国圆田螺在干旱休眠时与正常有水养殖相比，其肾组织中110个基因上调，389个基因下调；在肝组织中有84个基因上调，86个基因下调。肝组织差异基因主要与细胞黏附的调节、细胞外基质组织生长、神经元投射和轴突再生等功能相关；肾组织差异基因与碳水化合物代谢过程、去磷酸化、上皮细胞增殖调节、羟酸有机酸跨膜转移、组织重塑等功能相关；KEGG富集分析发现，差异基因主要定位在PI3K-Akt信号通路、蛋白聚糖、乳糖、鞘脂的合成代谢通路等与干旱胁迫相关的主要代谢通路上。研究表明，热激蛋白基因Hsp70、SRCR、FASN、APMAP、MSTN、Poc1b、S1P、Na+-K+-ATP酶β1亚基及SLC28A3基因在中国圆田螺干旱休眠时开启自身调节，田螺通过调控不同基因，启动阻隔自身对外界光线的感知，停止脂肪积累、抑制肌肉生长、吞噬自身凋亡细胞获取营养、调节细胞渗透压保水等使自身快速适应干旱休眠状态。 |
| 英文摘要: |
| [Objectives] Cipangopaludina chinensis is widely distributed in Asia. It has strong adaptability and can quickly enter a dormancy state under drought.In this paper, we study the dormancy characteristic of C. chinensis under drought stress to provide a research clue for revealing the mechanism of extensive adaptive tolerance, as well as the genetics and breeding of the C. chinensis. [Methods] The samples of C. chinensis were collected in Yancheng. Select individuals with sound ontogeny and consistent size to temporarily keep in the laboratory for 2 days. 30 C. chinensis were placed in an anhydrous plastic bucket for drought stress treatment, and the room temperature was adjusted to 24 ℃. 30 C. chinensis were placed in plastic buckets with water as control, and kept temporarily for 30 days. After 30 days, the liver and kidney tissues of the control group and the stress group were taken at random for 3 biological replications. High throughput sequencing technology was used to sequence and analyze the transcriptome of the liver and kidney tissues of C. chinensis under drought stress. After cleaning the raw sequencing datathen we identified different expressed genes by P < 0.05 and | log2FoldChange | > 2. Finally, GO (gene ontology) annotation and pathway enrichment analysis of the different expressed geneshave been done. [Results] The results showed that under drought stress, 110 genes were up-regulated and 389 genes were down regulated in the kidney tissue, 84 genes were up-regulated, and 86 genes were down-regulated in the liver tissue (Fig. 3), gene ontology is mainly related to the regulation of cell adhesion, extracellular matrix organization, neuron projection regeneration, carbohydrate metabolic process, dephosphorylation, regulation of epithelial cell proliferation, carboxylic acid, organic acid transmembrane transport, and tissue remodeling (Fig. 5); the KEGG enrichment analysis showed that the differently expressed genes were mainly enriched in PI3K-Akt signal pathway, proteoglycan, lactose, sphingolipid biosynthesis and metabolic pathway 5 that are the main pathways under drought stress (Fig. 6). [Conclusion] Based on the enrichment analysis results of GO and KEGG, heat shock protein genes Hsp70, SRCR, SLC28A3, FASN, APMAP, MSTN, Poc1b, S1P and Na+- K+- ATPase β Subunit 1 have been identified as important genes related to dormancy of the C. chinensis under drought stress. |
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