| 赵玉瑞,朱滨清,高朔,王乐,吴延庆.2023.浙江庆元发现长肢林蛙.动物学杂志,58(4):607-614. |
| 浙江庆元发现长肢林蛙 |
| Long-Legged Brown Frog Rana longicrus Found in Qingyuan,Zhejiang |
| 投稿时间：2022-12-09 |
| DOI：10.13859/j.cjz.202304013 |
| 中文关键词: 长肢林蛙 浙江省 庆元县 新记录种 |
| 英文关键词:Rana longicrus Zhejiang Province Qingyuan County New record |
| 基金项目:国家自然科学基金项目(No. 32001111)，庆元县生物多样性保护与可持续项目-生态系统和生物资源本底调查与评估； |
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| 中文摘要: |
| 2022年10月在浙江省庆元县贤良镇采集到5只林蛙属(Rana)雌性个体，经形态和分子系统发育分析鉴定为长肢林蛙(R. longicrus)，是浙江省两栖动物分布新记录种。所采集的5只长肢林蛙个体体长40.4 ~ 55.2 mm，头长大于头宽，肩部上方由数个疣粒隐约组成“八”字形黑斑；背侧褶明显，与胯部相连；四肢背面具黑褐色横纹，后肢前伸贴体时胫跗关节超过吻端，形态特征与地模标本基本一致。基于线粒体16S rRNA基因片段进行系统发育分析表明，该蛙与模式产地中国台湾的长肢林蛙序列聚成单系支，且遗传距离小于0.005%。此前记录的长肢林蛙仅分布于我国台湾、广东、福建、江西等地，长肢林蛙在浙江省庆元县的分布为浙江首次记录，这提示该蛙有更广的分布范围。 |
| 英文摘要: |
| During the investigation on amphibians in October 2022, 5 female specimens of Rana (Anura:Ranidae) were collected from Xianliang Town, Qingyuan County, Zhejiang Province, China. We compared their features by morphology and phylogeny and identified them as R. longicrus. This represents the first record of this species in Zhejiang Province. 5 female samples collected from Qingyuan County were measured with electronic vernier calipers. The measurements were:snout-vent length, head length, head width, snout length, internasal space, interorbital space, diameter of eye, length of lower arm and hand, tibia length, foot length, and hindlimb length. We compared these morphological characteristics with the specimens from Fujian, Jiangxi, Guangdong, and Taiwan, China. In this study, phenol/chloroform extraction was used to extract genomic DNA from the hindlimb muscle tissue of Brown Frogs collected in Qingyuan County, Zhejiang Province. 16S rRNA gene was amplified, and the primers were 5′-CGC CTG TTT ACC AAA AAC AT-3′ and 5′-CCG GTC TGA ACT CAG ATC ACG T-3′. Based on 16S rRNA gene fragments, we used Mrbayes-3.1.2 to construct Bayesian tree for phylogenetic analysis and used p-distance in MEGA 7 to calculate the genetic distance between species of Rana. All the new sequences in this research have been uploaded to the GenBank database. The 16S rRNA sequences of 16 species of Rana distributed in East China and Glandirana tientaiensis were obtained from GenBank (Table 2).The morphological characteristics are consistent between the specimens collected in this study and the topotype of R. longicrus. Our specimens have a medium snout-vent length (40.4 to 55.2 mm); head length is larger than the head width; some sparse granules can form a V-shaped figure on the shoulder; dorsolateral fold obvious and connect with the crotch; arms and legs with dark brown bands; tibiotarsal articulation beyond snout. In addition, the Bayesian analyses based on 16S rRNA strongly support the specimens we collected form a monophyletic clade with the topotype specimens (Fig 2), and the genetic distance is less than 0.005% between our specimens and topotype (Table 3).The specimens collected in Qingyuan County are determined to be R. longicrus, which is the first record of this species in Zhejiang Province. In this study, we further updated the distribution range of R. longicrus. |
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