| 杨喜喜,铁雅楠,王志亮,伏晓玉,王欣荣.2023.不同剂量孕马血清促性腺激素超排处理对家兔卵巢组织形态和胚胎发育的影响.动物学杂志,58(4):597-605. |
| 不同剂量孕马血清促性腺激素超排处理对家兔卵巢组织形态和胚胎发育的影响 |
| Effects of Different Doses of Pregnant Mare Serum Gonadotropin on Ovary Morphology and Embryonic Development in Rabbits |
| 投稿时间：2022-03-15 |
| DOI：10.13859/j.cjz.202304012 |
| 中文关键词: 卵巢 卵泡 胚胎 生殖激素 家兔 |
| 英文关键词:Ovarian Follicles Embryos Reproductive hormones Rabbits |
| 基金项目:甘肃农业大学特色学科开放基金项目(No. GAU-XKJS-2018-037)； |
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| 中文摘要: |
| 为了研究不同剂量的孕马血清促性腺激素(PMSG)对家兔(Oryctolagus cuniculus f. domesticus)超排后，卵巢组织形态和PMSG对早期胚胎体外发育的影响。将24只家兔分为对照组、50 IU、70 IU和90 IU组，每组6只，对照组不做处理，后3组分别注射50 IU、70 IU和90 IU PMSG和定量100 IU人绒毛膜促性腺激素(HCG)对家兔进行超排处理。解剖后测定卵巢形态和组织参数。利用注射器抽取5 ml冲卵液，分别从左右输卵管冲取胚胎至培养皿中，转至细胞培养室对胚胎进行体外培养观察。结果显示，PMSG处理组与对照组相比，随着PMSG注射剂量的增大，家兔卵巢重及宽度和厚度均极显著增大(P < 0.01)，卵巢长度显著增长(P < 0.05)，卵巢表面充血，卵泡数增多；另外，随PMSG注射剂量的增大，次级卵泡及其卵母细胞直径、三级卵泡直径均呈减小趋势(P < 0.01)，三级卵泡中卵母细胞直径呈增大趋势，与对照组相比差异显著(P < 0.05)；三级卵泡的直径和卵泡的优势化率，70 IU组显著高于对照组及50 IU和90 IU组(P < 0.05)。90 IU的PMSG会导致超排的不良影响显现，而70 IU的PMSG可促进三级卵泡中卵母细胞的发育、各级卵泡的数目增多及优势化率增高，但同时也减小了各级卵泡的直径。胚胎体外培养时，PMSG实验组与对照组相比，因PMSG注射剂量的增大，胚胎畸形率极显著增高(P < 0.01)，2-细胞率极显著下降(P < 0.01)，4-细胞率显著下降(P < 0.05)。 |
| 英文摘要: |
| [Objectives] In order to study the effects of different doses of pregnant mare serum gonadotropin (PMSG) on ovarian morphology and early embryo development in vitro after superovulation in rabbits (Oryctolagus cuniculus f. domesticus). [Methods] Twenty-four rabbits were randomly divided into four groups, with 6 rabbits in each group. The control group was not treated. The latter three groups were injected with 50 IU, 70 IU and 90 IU PMSG , and 100 IU human chorionic gonadotropin (HCG) for superovulation. After dissection, the morphology and tissue parameters of ovaries were measured. Embryos were flushed from the left and right Fallopian tubes into Petri dishes by using 5 ml washing solution and then transferred to the cell culture room for in vitro culture and observation. The results were expressed as “Mean ± standard deviation, and the data were analyzed by one-way ANOVA with SPSS 25.0. P < 0.01 means the difference is extremely significant, P < 0.05 means the difference is significant, and P ≥ 0.05 means the difference is not significant. [Results] The results showed that compared with the control group, the weight, width, and thickness of ovaries in the PMSG-treated group increased extremely significantly (P < 0.01), the length of ovaries increased significantly (P < 0.05), the surface of ovaries became congested, and the number of follicles increased with the increase of PMSG injection dose (Fig. 1, Table 1). In addition, with the increase of PMSG injection dose, the diameters of secondary follicles and their enclosed oocytes, and the diameter of tertiary follicles decreased (P < 0.01), while the diameter of oocytes in tertiary follicles increased, with a significant difference compared with the control group (P < 0.05, Fig. 3, Table 3). The diameter of tertiary follicles and the dominance rate of follicles in the 70 IU group were significantly higher than those in the control group, 50 IU group, and 90 IU group (P < 0.05, Table 4). When the embryos were cultured in vitro, compared with the control group, PMSG treated group had a significantly higher embryo deformity rate (P < 0.01), significantly lower 2-cell rate (P < 0.01), and significantly lower 4-cell rate (P < 0.05, Fig. 2, Table 2) due to the increase of PMSG injection dose. [Conclusion] 90 IU PMSG superovulation can cause adverse effects, while 70 IU PMSG can increase the numbers of follicles at all levels and the dominance rate, and promote the development of oocytes in tertiary follicles, but reduce the diameters of follicles at all levels. |
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