| 徐文秀,刘庆玲,刘笑含,孔令英,董思林,许文达,石慧,赵振军.2023.乙醛脱氢酶基因ALDH2对雄性小鼠生育能力的影响.动物学杂志,58(4):590-596. |
| 乙醛脱氢酶基因ALDH2对雄性小鼠生育能力的影响 |
| The Effects of Acetaldehyde Dehydrogenase Gene ALDH2 on Male Mice Fertility |
| 投稿时间：2022-09-19 |
| DOI：10.13859/j.cjz.202304011 |
| 中文关键词: 乙醛脱氢酶2 睾丸 精子发生 生育能力 |
| 英文关键词:Aldehyde Dehydrogenase 2 Testis Spermatogenesis Fertility |
| 基金项目:国家重点研发计划项目(No. 2018YFC1003600)； |
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| 摘要点击次数: 56 |
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| 中文摘要: |
| 本研究使用乙醛脱氢酶2(ALDH2)基因全敲除的雄性C57BL/6(B6)小鼠(Mus musculus domesticus)，通过分析不同周龄小鼠的睾丸脏器系数、睾丸组织细胞形态、精子运动参数、配种后母鼠产仔数及子代雄雌比等生育指标，探讨ALDH2基因敲除对雄性小鼠生育能力的影响。结果表明，与野生型(WT)小鼠相比，5、7、10周龄ALDH2基因敲除型(KO)雄性小鼠睾丸脏器系数显著降低(P < 0.05)；睾丸组织细胞间质变大，精子活率显著降低(P < 0.05)；产仔数和雄雌比显著降低(P < 0.05)。本研究为揭示乙醛脱氢酶ALDH2基因在雄性小鼠生殖中的作用提供了一定的基础。 |
| 英文摘要: |
| [Objectives] The research on mammalian sexual reproduction is beneficial to the exploration of human reproduction mechanism, which focuses on reproductive system, germ cells, fertilization cells, external environmental factors, etc. There is little research on mammalian fertility after the elimination of metabolic enzymes from the body. It is known that aldehyde dehydrogenase 2 (ALDH2) is the most important enzyme for aldehyde oxidation, which can metabolize a large number of harmful aldehydes produced in the body. Studies have shown that a large number of reactive oxygen species produced by male sperm during exercise can increase the aldehyde content in sperm. Therefore, we studied the fertility of ALDH2 gene knockout male mice. [Methods] In this study, we analyze the testicular organ coefficient, testicular tissue cell morphology, sperm motility parameters, the number of offspring born after mating, and the ratio of male to female offspring of 52 male C57BL/6 mice of different weeks of age with ALDH2 gene knockout. Mean value comparison, one-way ANOVA, and t-test implemented in SPSS were used to explore the effect of ALDH2 gene knockout on fertility of male mice. [Results] We found that compared with the wild type (WT) mice, the weight and testicular organ coefficient of knockout (KO) mice at 5 and 10 weeks of age and mice at 3 and 10 weeks of age had extremely significant differences (n = 6, P < 0.01) (Table 1), the weight of mice at 3 and 7 weeks of age and testicular organ coefficient of mice at 5 and 7 weeks of age had significant differences (n = 6, P < 0.05) (Table 1). Among them, the cytoplasm of testicular tissue of 10 week old ALDH2 gene knockout male mice became larger (Fig. 2b); the sperm activity rate of knockout mice was significantly reduced (n = 6, P < 0.05) (Table 2); the litter size of ALDH2 transgenic mice was significantly reduced (6.7 ± 0.5) compared with wild type mice (11.0 ± 2.0, n = 3, P < 0.05), and the female to male ratio of ALDH2 gene knockout mice was also significantly reduced (0.8 ± 0.1 vs. 1.8 ± 0.5, n = 3, P < 0.05). [Conclusion] This study provides a basis for revealing the role of ALDH2 gene in male mouse reproduction and provides valuable information for the potential diagnosis and in vitro application of assisted reproductive technology. |
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