| 程明阳,张勘,梁福轩,何流洋,朱玉东,虞皓琦,肖璐瑶,郑普阳,谢锋.2023.不同取样方式下大凉螈精子形态变化.动物学杂志,58(4):554-562. |
| 不同取样方式下大凉螈精子形态变化 |
| Sperm Morphological Change of Liangshantriton taliangensis Under Different Sampling Methods |
| 投稿时间：2022-12-06 |
| DOI：10.13859/j.cjz.202304008 |
| 中文关键词: 大凉螈 精子取样方式 精子形态 精子修饰 |
| 英文关键词:Liangshantriton taliangensis Sperm sampling methods Sperm morphology Sperm modification |
| 基金项目:国家重点研发计划重点专项(No. 2017YFC0505202)，四川省科技重大专项(No. 2018SZDZX0036)，生态环境部生物多样性保护专项(No. 2111101)，四川栗子坪国家级自然保护区物种保护项目(No. 2022)； |
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| 中文摘要: |
| 精子形态在精子竞争和精子进化中具有重要地位。体内受精有尾两栖类雌性和雄性均具有复杂的泄殖腔腺体，都可能对精子形态进行进一步包装和修饰，但这些过程对精子形态特征和功能的影响目前所知甚少。本研究以体内受精的大凉螈(Liangshantriton taliangensis)为研究对象，系统观察了破碎睾丸、雄性自然排精和冲洗雌性泄殖腔3种方法所收集精子的形态变化；同时，分别利用了t检验、t’检验或U检验，探究了其定量变化。结果显示：取自精子囊和雌性泄殖腔的精子可观察到明显的颈部，而破碎睾丸取样的精子仅观察到胞质小团结构，无明显颈部结构，故推测睾丸中精子尚未成熟；精子在由睾丸到排出体外过程中顶体显著缩短，尾部所占比例显著增加(U检验或t检验，P < 0.05)，而精子在由精子囊到进入雌性体内的过程中未发生形态或量度上的显著变化(U检验或t检验，P > 0.05)，仅表现为顶体的进一步缩短(t检验，P < 0.05)。本研究提示，精子由睾丸到排出体外的过程中可能因其经历的修饰过程而展现出形态和量度上的差异，为精子生物学研究和精子保存提供了选择依据。 |
| 英文摘要: |
| [Objectives] Sperm morphology is important in sperm competition and sperm evolution. The complex cloacal glands in both males and females of caudate amphibians with internal fertilization may be responsible for further packaging and modification of spermatogenesis, but little is known about the effects of these processes on sperm morphology and function. [Methods] In this study, the species Liangshantriton taliangensis which practices internal fertilization by transferring a spermatophore was studied. The morphological changes of the features of spermatozoa collected by 3 kinds of sampling methods (testes crashing, spermatophore, and female cloaca flushing) were systematically compared. Using t-test, t’-test, or Mann-Whitney U test, we analyzed the sperm morphological changes, and significant differences were set at P < 0.05. [Results] The sperm from the spermatophore and female cloaca flushing had a distinct neck, while the sperm from the testes had no such structure, but with cytoplasmic masses around the sperm head (Fig. 1). In addition, sperm shortens the acrosome and increases the proportion of sperm tail in the process of being passed out of the body by the testes (Mann-Whitney U test or t-test, P < 0.05) (Table 1), no significant change (Mann-Whitney U test or t-test, P > 0.05) in sperm morphology and measurement during the process from spermatophore to female body except for the further shortening of acrosome (t-test, P < 0.05) (Table 2). [Conclusion] These results suggest that sperm samples from the testis, spermatophore, or female ejaculatory cavity may show morphological and functional differences due to modification processes, which provide great options for the sampling method for the sperm biology or sperm conservation studies. |
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