| 段爱丽,陈昆慈,张新铖,李伟,朱阿莉,洪孝友,朱新平.2014.斑鳢、乌鳢及其杂种细胞核DNA流式含量分析.动物学杂志,49(1):46-50. |
| 斑鳢、乌鳢及其杂种细胞核DNA流式含量分析 |
| Cellular DNA Contents of Channa maculata, C.argus and Their Reciprocal Hybrids Determined by Flow Cytometry |
| 投稿时间：2013-06-06 修订日期：2013-10-31 |
| DOI： |
| 中文关键词: 斑鳢 乌鳢 杂种 流式细胞术 DNA含量 |
| 英文关键词:Channa maculata C.argus Reciprocal hybrids Flow ctyometry DNA contents |
| 基金项目:国家科技支撑计划课题（No.2012BAD26B03）；国家公益性行业（农业）科研专项（No.200903045） |
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| 中文摘要: |
| 以斑鳢（Channa maculata）、乌鳢（C.argus）及其正交杂种斑乌鳢（斑鳢♀×乌鳢♂）和反交杂种乌斑鳢（乌鳢♀×斑鳢♂）的红细胞为材料，以鸡（Gallus gallus）血细胞为DNA标准（2.5 pg/2c，2c指2倍体），采用流式细胞仪测定了这4种鱼的细胞核DNA含量。斑鳢、乌鳢、斑乌鳢及乌斑鳢这4种鱼血细胞DNA的绝对含量分别为（1.488±0.035）pg/2c、（1.489±0.034）pg/2c、（1.522±0.077）pg/2c和（1.520±0.033）pg/2c。斑鳢和乌鳢的细胞核DNA含量差异不显著（P>0.05），斑鳢和乌鳢与两种杂交鳢的DNA含量差异显著（P<0.05），两种杂交鳢之间的细胞核DNA含量差异不显著（P>0.05）。杂交鳢细胞核DNA含量显著高于亲本，可以作为杂种鉴定的依据。 |
| 英文摘要: |
| The cellular DNA contents of Channa maculata, C.argus and their reciprocal hybrids (C.maculata ♀×C.argus ♂, C.argus ♀×C.maculata ♂) were assayed by using a flow cytometer with the DNA of chicken red blood cells (2.5 pg/2c, 2c:diploid) as a standard. The DNA contents of the 4 species of fish are 1.488±0.035 pg/2c (C.maculata ♀), 1.489±0.034 pg/2c (C.argus ♂), 1.522±0.077 pg/2c (C.maculata ♀×C.argus ♂), and 1.520±0.033 pg/2c (C.argus ♀×C.maculata ♂) respectively. Comparing the DNA contents of the 4 species of fish, it was showed that there was no significant difference between C.maculata and C.argus (P>0.05), and the similar result was obtained between their reciprocal hybrids (P>0.05), but there was significant difference between reciprocal hybrids and their parents (P<0.05). The DNA contents of the reciprocal hybrids are more than those of their parents, which may be used for hybrid identification. |
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