Acoustic signal is important on animal communion. Using vocalizations of animal to locate the position of individuals is a non-invasive method, and it is more feasible to monitor the wildlife that inhabit thick vegetation or mainly active at night. The possibility of acoustic location was tested in field with songs of passerine birds in this study. The field simulation experiment was conducted in the Dongzhai National Nature Reserve, Henan province during April and May in 2013. The experiment was conducted with 4 stereo recorders placed at the four corners of boundary of 4 square simulant territories of dense shrubs. The songs of 5 species of songbirds were played back at the sites that were selected randomly in the squares. With exploiting differences in sound arrival times at each recorder, we calculated the positions of acoustic sources, and compared them with the actual positions. The mean accuracy across five species was 1.89±0.99 m. We concluded that this system produced accurate location estimates based on multi-channel recordings of some types of acoustic signals. This study introduced a new technology and it might be useful to wildlife researchers.